Understanding and Controlling Biomolecular Structure and Function at Surfaces and Interfaces

了解和控制表面和界面的生物分子结构和功能

• 批准号: 1361252
• 负责人: Lauren Webb
• 金额: $ 36.85万
• 依托单位: University of Texas at Austin
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-15 至 2017-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1361252&HistoricalAwards=false
• 关键词: Understanding; Controlling; Biomolecular; Structure; Function

With this new award, the Chemical Structure, Dynamics and Mechanisms A Program (CSDM-A) and the Chemistry of Life Processes Program (CLP) of the Division of Chemistry support Professor Lauren Webb of the University of Texas at Austin in a project that will investigate new methods for attaching proteins to surfaces under conditions that allow the protein activity to be studied. These studies, which will allow construction of surfaces with functional proteins attached, will have applications in material science and biomedical engineering. This project, which combines biophysics, molecular biology with surface and materials chemistry, will provide multidisciplinary training to students, from undergraduates to post-doctorals, including those from groups historically underrepresented in the sciences.Prof. Webb and her group are conducting a multi-pronged effort with the aim of developing surfaces that mimic stable and functional biological materials capable of both natural and artificial functions. Specific experiments that are being conducted include: (1) the further work on the chemical functionalization of alkane thiol surfaces with peptides, with engineered secondary structure; (2) the binding of more complex protiens to the protein surfaces created in (1); (3) the use of surface functionalization developed in (1) to drive the formation of protein aggregates on the surface; (4) the use of protein-functionalized surfaces to bind functional forms of enzymes to the surface and to measure their activity. Students working with Prof. Webb are obtaining significant experience in analytical, physical and biochemical methods. This interdisciplinary training prepares them for work in the technical workforce.

有了这个新奖项，化学结构，动力学和机制计划（CSDM-A）和化学过程的化学过程计划（CLP），化学工艺部支持德克萨斯大学奥斯汀分校的劳伦·韦伯教授劳伦·韦伯教授，该项目将在一个项目中研究在允许研究蛋白活性的条件下，将蛋白质连接到表面的新方法。这些研究将允许使用附有功能蛋白的表面构建，将在材料科学和生物医学工程中应用。该项目将生物物理学，分子生物学与表面和材料化学结合在一起，将为从本科生到博士后的学生提供多学科培训，包括科学中历史上代表性不足的群体的培训。韦伯和她的小组正在进行多管齐下的努力，目的是开发表面，以模仿能够自然和人工功能的稳定和功能性生物学材料。 正在进行的特定实验包括：（1）用肽和工程二级结构对烷烃硫醇表面化学功能化的进一步工作； （2）更复杂的蛋白质与（1）中产生的蛋白质表面的结合； （3）在（1）中开发的表面功能化的使用来驱动表面上的蛋白质聚集体的形成； （4）使用蛋白质官能化表面将酶的功能形式结合到表面并测量其活性。 与Webb教授一起工作的学生正在获得分析，物理和生化方法的重要经验。 这种跨学科培训为他们准备在技术劳动力中工作做好准备。