STTR Phase I: Robust, portable holographic imaging for label-free analysis of blood samples in the field

STTR 第一阶段：强大的便携式全息成像，用于现场血液样本的无标记分析

• 批准号: 1346349
• 负责人: William Brown
• 金额: $ 22.5万
• 依托单位: M2 Photonics Innovations
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-01-01 至 2014-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1346349&HistoricalAwards=false
• 关键词: STTR; Phase; Robust; portable; holographic

This Small Business Technology Transfer (STTR) Phase I project seeks to develop a novel holographic microscope system for examining cytological samples, such as blood smears, at the point of care. Current blood cell analysis techniques require manual examination by expert technicians, resulting in a costly and time-consuming test. The project proposes to develop a compact, portable, inexpensive, and easy-to-use wide field digital holographic microscope for automated screening of blood and cell samples. In this unique microscope design, optical fidelity and alignment are achieved through the use of an interferometric sample chamber, which produces holographic images of cell samples with simple laser illumination. The objectives of this project are to build a prototype microscope with low-cost chambers capable of imaging 1 million cells in 5 minutes.The broader impact/commercial potential of this project is as new tool for detection of diseases such as malaria. Even with recent advances, malaria is a significant health concern with 300-500 million cases each year and 1-3 million deaths, 90% of which occur in impoverished regions in sub-Saharan Africa with the majority of the cases occurring in young children. The proposed holographic microscope can provide rapid diagnosis for far less expense than manual examination of blood smears, while improving on the accuracy of comparably priced antigen based tests. The commercial opportunity is significant both in the U.S. and abroad. Malaria detection is the first target; there are additional opportunities for detection of other blood based diseases as the technology develops.

该小型企业技术转让 (STTR) 第一阶段项目旨在开发一种新型全息显微镜系统，用于在护理时检查细胞学样本，例如血涂片。目前的血细胞分析技术需要专业技术人员进行手动检查，导致测试成本高昂且耗时。该项目拟开发一种紧凑、便携、廉价且易于使用的宽视场数字全息显微镜，用于自动筛选血液和细胞样本。在这种独特的显微镜设计中，通过使用干涉样品室实现光学保真度和对准，该样品室通过简单的激光照明产生细胞样品的全息图像。该项目的目标是构建一个具有低成本腔室的原型显微镜，能够在 5 分钟内对 100 万个细胞进行成像。该项目更广泛的影响/商业潜力是作为检测疟疾等疾病的新工具。即使最近取得了进展，疟疾仍然是一个重大的健康问题，每年有 300-5 亿病例和 1-300 万人死亡，其中 90% 发生在撒哈拉以南非洲的贫困地区，其中大多数病例发生在幼儿中。所提出的全息显微镜可以提供快速诊断，其费用远低于手动检查血涂片，同时提高了价格相当的基于抗原的测试的准确性。无论是在美国还是在国外，商业机会都很重要。疟疾检测是首要目标；随着技术的发展，还有更多机会检测其他血液疾病。