SBIR Phase II: Widefield Three-Dimensional Superresolution Microscopy Module

SBIR 第二阶段：宽视场三维超分辨率显微镜模块

• 批准号: 1353638
• 负责人: Anurag Agrawal
• 金额: $ 62.23万
• 依托单位: Double Helix Optics Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-01 至 2017-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1353638&HistoricalAwards=false
• 关键词: SBIR; Phase; II; Widefield; Three

This Small Business Innovation Research (SBIR) Phase II project is focused on the design, development, and testing of a ground-breaking three-dimensional (3D) multifunctional quantitative optical microscopy module with single-molecule sensitivity suitable for live cell studies and nanometer scale sensing. In spite of the current revolution in optical microscopy, commercial solutions to achieve resolution beyond the diffraction limit have been slow to market, lacking in flexibility, and costly. Furthermore, most commercial products are too tailored to the systems being investigated and are difficult to generalize for widespread use. The instrument to be developed is based on an integrated design of the illumination, optics, data collection, and reconstruction algorithms for fluorescence imaging. These techniques enable the determination of the 3D position of emitters with great precision leading to 3D imaging with better resolution and depth of field than competing methods. Experimental results have consistently shown 3D capability with resolutions below 20 nm, representing one order of magnitude improvement over optical microscopes currently in use at most research institutions.The broader impact/commercial potential of this project addresses a major opportunity in the expanding microscopy market. The module's unique characteristics and accessible price point will offer multifunctional 3D superresolution imaging capability to thousands of laboratories in the US and worldwide. In an effort to accelerate commercialization, the project seeks to implement a cost-effective, flexible modular subsystem that can be integrated with existing commercial scientific microscopes. With its widespread accessibility, the instrument will impact multiple fields of science and engineering including biophysics, cellular biology, and biomedical science and engineering. From a broader perspective, this project will increase scientific and technical capabilities by providing nanoscale optical imaging for everyday biological research, thereby strengthening the US presence in the worldwide optical microscopy industry.

这项小型企业创新研究（SBIR）II期项目的重点是针对实用细胞研究和纳米量表感测的单分子灵敏度，具有针对单分子灵敏度的突破性三维（3D）多功能定量光学显微镜模块的设计，开发和测试。尽管当前的光学显微镜进行了革命，但在衍射限制之外实现解决方案的商业解决方案的市场却很慢，缺乏灵活性和昂贵。此外，大多数商业产品对正在研究的系统的量身定制，并且很难普遍使用。要开发的仪器是基于用于荧光成像的照明，光学，数据收集和重建算法的集成设计。这些技术能够确定发射器的3D位置，其精确度很高，从而使3D成像具有比竞争方法更好的分辨率和景深。 实验结果始终显示出3D能力，分辨率低于20 nm，这比目前大多数研究机构使用的光学显微镜的一个数量级改进。该项目的更广泛的影响/商业潜力解决了不断扩展的显微镜市场的主要机会。该模块的独特特征和可访问的价格点将为美国和全球的数千个实验室提供多功能的3D超分辨率成像能力。为了加速商业化，该项目试图实施一个具有成本效率，灵活的模块化子系统，该系统可以与现有的商业科学显微镜集成。该乐器将凭借其广泛的可访问性影响科学和工程的多个领域，包括生物物理学，细胞生物学以及生物医学科学和工程。从更广泛的角度来看，该项目将通过为日常生物学研究提供纳米级光学成像来提高科学和技术能力，从而增强美国在全球光学显微镜行业中的存在。