CAREER: Development of a Multifunctional Polyester Platform and its Evaluation as an Instructive Matrix for Osteoblast Differentiation

职业：多功能聚酯平台的开发及其作为成骨细胞分化指导基质的评估

• 批准号: 1352485
• 负责人: Abraham Joy
• 金额: $ 50万
• 依托单位: University of Akron
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-01 至 2020-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1352485&HistoricalAwards=false
• 关键词: CAREER; Development; Multifunctional; Polyester; Platform

Non-technical Abstract: This CAREER Award by the Biomaterials program in the Division of Materials Research, and co-funded by the Biomedical Engineering Program in the Division of Chemical, Bioengineering, Environmental and Transport Systems (ENG/CBET) to the University of Akron is to develop a modular multifunctional polyester platform with peptide-like functional groups to be used as a matrix for stem cell differentiation. Products derived from polymeric materials are an integral part of modern life and polymer science has addressed several technological challenges. In addition to their extensive use as commodity and engineering materials, polymers are also widely used in the biomedical field to make implants, sutures, medical catheters, wound healing products etc. In spite of such extensive use, the design and performance of such synthetic materials lags far behind in their ability to match the performance of natural materials such as collagen in providing the functional cues necessary for optimum biological outcomes, such as in wound healing or precise growth and maturation of new tissue. The project will identify the key differences between natural materials such as collagen and widely used synthetic materials and offers a bottom-up design of a synthetic polymeric material platform. This modular synthetic polymer system, when fully developed, will have the physical and biological properties that would enable their use in several biomedical applications such as for differentiation of stem cells, as antimicrobials and for drug/protein delivery. This CAREER award facilitates the training of the next generation of scientists in STEM fields and provides fundamental training at the interface of chemistry, materials science and biology. The award specifically provides research opportunities for high school, undergraduate and graduate students. Socially and economically disadvantaged students from local high schools will be mentored through this program. In addition a select group of high school students with strong credentials will have the opportunity to engage in cutting edge research. In addition, the award enables to create a suite of freely available online educational videos describing the fundamental principles of polymer science.Technical Abstract: The key transmitter of information between cells and their surroundings is the extracellular matrix (ECM) and this information is processed by specific binding and signaling events between cells and the components of the ECM. Although synthetic biomaterials are being explored for an increasing number of clinical biomedical needs, the ability of current biomaterials to provide the required specificity in binding and signaling is still in its infancy due to the complexity in balancing the material parameters of functionality, reaction feasibility, degradability, hydrophilicity etc. The award will support the development of a new pendant functionalized polyester system which aims to provide the advantages of functionality and specificity as seen in peptides and proteins while also having the advantages of synthetic reproducibility and scalability that synthetic materials provide. As an illustrative case study, these multifunctional polyesters will be investigated as an instructive matrix for osteoblast differentiation with the aim of correlating the chemical profile of the multifunctional polyester system with stem cell proliferation and differentiation. Such a modular synthetic platform with the ability to precisely vary the polymer composition will be useful in several biomedical applications such as drug/protein delivery, self-assembled systems, multi-valent inhibitors and as antimicrobial agents. Students engaged in this research will work at the interface of chemistry, materials science and cell biology and will also have the opportunity to understand how their research addresses fundamental needs in the biomedical sciences.

非技术摘要：该职业奖由材料研究部生物材料项目颁发，并由阿克伦大学化学、生物工程、环境和运输系统 (ENG/CBET) 部生物医学工程项目共同资助的目的是开发一种模块化多功能聚酯平台，其具有肽样官能团，可用作干细胞分化的基质。源自聚合物材料的产品是现代生活不可或缺的一部分，聚合物科学已经解决了多项技术挑战。除了广泛用作商品和工程材料外，聚合物还广泛用于生物医学领域，以制造植入物、缝合线、医用导管、伤口愈合产品等。尽管用途广泛，但此类合成材料的设计和性能在提供最佳生物学结果（例如伤口愈合或新组织的精确生长和成熟）所需的功能线索方面，它们的性能远远落后于胶原蛋白等天然材料的性能。该项目将确定胶原蛋白等天然材料与广泛使用的合成材料之间的主要区别，并提供合成聚合物材料平台的自下而上的设计。这种模块化合成聚合物系统在完全开发后，将具有物理和生物特性，使其能够用于多种生物医学应用，例如干细胞分化、抗菌剂和药物/蛋白质输送。该职业奖促进了 STEM 领域下一代科学家的培训，并提供化学、材料科学和生物学交叉领域的基础培训。该奖项专门为高中生、本科生和研究生提供研究机会。来自当地高中的社会和经济弱势学生将通过该计划得到指导。此外，一批具有良好资历的高中生将有机会参与前沿研究。此外，该奖项还能够创建一套免费的在线教育视频，描述聚合物科学的基本原理。 技术摘要：细胞与其周围环境之间信息的关键传递者是细胞外基质（ECM），该信息由细胞和 ECM 成分之间的特异性结合和信号传导事件。尽管合成生物材料正在被探索以满足越来越多的临床生物医学需求，但由于平衡功能性、反应可行性、可降解性等材料参数的复杂性，当前生物材料提供所需的结合和信号传导特异性的能力仍处于起步阶段。该奖项将支持新型悬垂功能化聚酯系统的开发，该系统旨在提供肽和蛋白质中所见的功能性和特异性优势，同时还具有合成再现性和可扩展性的优势。材料提供。作为一个说明性案例研究，这些多功能聚酯将作为成骨细胞分化的指导基质进行研究，目的是将多功能聚酯系统的化学特征与干细胞增殖和分化相关联。 这种能够精确改变聚合物组成的模块化合成平台将可用于多种生物医学应用，例如药物/蛋白质递送、自组装系统、多价抑制剂和抗菌剂。从事这项研究的学生将在化学、材料科学和细胞生物学的交叉领域工作，还将有机会了解他们的研究如何满足生物医学的基本需求。

项目成果

Opposing Effects of Side-Chain Flexibility and Hydrogen Bonding on the Thermal, Mechanical, and Rheological Properties of Supramolecularly Cross-Linked Polyesters

侧链柔性和氢键对超分子交联聚酯的热、机械和流变特性的相反影响

• DOI: 10.1021/acs.macromol.8b01781
• 发表时间: 2018-11-27
• 期刊: MACROMOLECULES
• 影响因子: 5.5
• 作者: Liu, Qianhui;Wang, Chao;Joy, Abraham
• 通讯作者: Joy, Abraham