Collaborative Research: Exploiting the Syntegron Technology Platform for Assembly and 0ptimisation of Complex Genetic Ensembles

合作研究：利用 Syntegron 技术平台进行复杂遗传集成体的组装和 0 优化

• 批准号: 1341414
• 负责人: Joshua Leonard
• 金额: $ 67.74万
• 依托单位: Northwestern University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1341414&HistoricalAwards=false
• 关键词: Collaborative; Research; Exploiting; Syntegron; Technology

Engineered microorganisms may serve as factories to convert renewable starting materials, such as sugars and plant-derived biomass, into valuable products including chemicals, fuels, and medicines. Although the feasibility and utility of this approach is now well established, engineering microorganisms and plants to produce novel products through the introduction of specific genes remains technically challenging and resource-intensive. Consequently, these barriers to bringing new biological synthesis platforms to commercial feasibility and efficiency limit the rate at which new technologies create public benefits and economic impacts. Moreover, it is not yet possible to efficiently harness sources of vast biodiversity, such as libraries of genetic information from plants and communities of organisms, because identifying and utilizing genetic "diamonds in the rough" remains too costly and technically intensive to conduct in most laboratories. To meet these needs, this project will develop a technology suite that enables researchers to efficiently assemble, evaluate, and optimize novel biological synthesis systems by harnessing evolutionary mechanisms to both generate and select for functional assemblies of genetic parts. This work includes novel approaches for identifying promising genes from plant genomes, biological sensors that enable engineers to monitor and control biomanufacturing within cells, and computational design tools that will enable the broader research community to apply these capabilities to a broad range of applications. Broader Impacts: Together, this work will create tools that catalyze the development of sustainable biological manufacturing platforms and enable the production of new medically and industrially useful molecules, thus contributing significantly to the Nation's economy. The project will also provide multi-disciplinary training of students and postdoctoral researchers in a rapidly emerging field.

工程的微生物可以用作工厂，将糖和植物衍生的生物量等可再生的起始物质转换为有价值的产品，包括化学物质，燃料和药品。尽管现在已经确定了这种方法的可行性和实用性，但通过引入特定基因的引入技术和资源密集型的工程微生物和植物来生产新产品。因此，这些将新的生物合成平台带到商业可行性和效率的障碍限制了新技术创造公共利益和经济影响的速度。此外，尚不可能有效利用巨大生物多样性的来源，例如来自生物体的植物和社区的遗传信息库，因为识别和利用遗传“粗糙的遗传“钻石”中的遗传“钻石”仍然太昂贵且在大多数实验室中进行了技术密集型。为了满足这些需求，该项目将开发一个技术套件，使研究人员能够通过利用进化机制来生成和选择遗传部位的功能组件来有效地组装，评估和优化新型的生物合成系统。这项工作包括从植物基因组中识别有前途的基因的新方法，使工程师能够监视和控制细胞内的生物制造以及计算设计工具，这些工具将使更广泛的研究社区能够将这些功能应用于广泛的应用。更广泛的影响：这项工作将创建工具，以促进可持续生物制造平台的发展，并能够生产新的医学和工业有用分子，从而对国家的经济产生重大贡献。该项目还将在快速新兴领域的学生和博士后研究人员提供多学科培训。