Mechanisms of neurogenesis in a segmented polychaete

分节多毛动物的神经发生机制

• 批准号: 1318772
• 负责人: Elaine Seaver
• 金额: $ 8.5万
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-12-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1318772&HistoricalAwards=false
• 关键词: Mechanisms; neurogenesis; segmented; polychaete

The central nervous system is a complex organ whose proper function is critical for coordination of locomotion, sensing and responding to environmental conditions, and is closely tied to animal survival. Understanding how such a complex organ develops represents a fundamental question in animal biology. Detailed cellular and molecular knowledge of neural development is limited to studies in model systems of two of the three major bilaterian clades, the ecdysozoans and deuterostomes. Capitella is a segmented worm and member of the third bilaterian clade, the lophotrochozoans. This project employs cellular, molecular and developmental approaches to characterize development of the centralized nervous system in this annelid model. Capitella offers several advantages for neurogenic studies, most notably that it undergoes both embryonic and adult neurogenesis, it exhibits robust adult regeneration and its genome has been fully sequenced. Early stages of brain development will be investigated using cell labeling methods, time-lapse video microscopy, and gene silencing techniques. The origin, specification, mode of internalization, and mitotic activity of neural progenitor cells of the Capitella nervous system will be determined. Molecular mechanisms controlling these processes will be analyzed using a combination of knockdown, mis-expression and pharmacological approaches. The project will provide crucial information of how the central nervous system is generated in polychaete annelids, fill a current gap in knowledge of the lophotrochozoans, and yield insights into the evolution of the centralized nervous system.The results of this project are of interest to several fields of biology including neurobiology, developmental biology and evolutionary biology. In addition, the proposed work will refine and further methodology for studying gene function in the model Capitella. The project will integrate training with research and through a commitment by the PI to training in the laboratory environment. Women and underrepresented groups such as Pacific Islanders will be encouraged to participate in the nation's scientific enterprise. Results will be disseminated through peer-reviewed publications, conference presentations and outreach activities to the local community.

中枢神经系统是一个复杂的器官，其正常功能对于协调运动、感知和响应环境条件至关重要，并且与动物的生存密切相关。 了解如此复杂的器官如何发育是动物生物学的一个基本问题。神经发育的详细细胞和分子知识仅限于对三个主要两侧对称动物分支中的两个（蜕皮动物和后口动物）模型系统的研究。 Capitella 是一种分节蠕虫，属于第三两侧对称动物分支，轮轮动物。该项目采用细胞、分子和发育方法来表征该环节动物模型中中枢神经系统的发育。 Capitella 为神经发生研究提供了多种优势，最值得注意的是它经历了胚胎和成体神经发生，它表现出强大的成体再生能力，并且其基因组已被完全测序。将使用细胞标记方法、延时视频显微镜和基因沉默技术来研究大脑发育的早期阶段。将确定小头神经系统神经祖细胞的起源、规格、内化模式和有丝分裂活性。将结合敲除、错误表达和药理学方法来分析控制这些过程的分子机制。该项目将提供关于多毛类环节动物中枢神经系统如何产生的重要信息，填补目前轮轮动物知识的空白，并深入了解中枢神经系统的进化。该项目的结果引起了一些人的兴趣。生物学领域包括神经生物学、发育生物学和进化生物学。此外，拟议的工作将完善和进一步研究 Capitella 模型中基因功能的方法。该项目将通过 PI 承诺在实验室环境中进行培训，将培训与研究结合起来。将鼓励妇女和太平洋岛民等代表性不足的群体参与国家的科学事业。结果将通过同行评审的出版物、会议演示和向当地社区的外展活动传播。