Tracking Cell Fate during Mechanically Directed Bone Regeneration In Vivo

体内机械定向骨再生过程中跟踪细胞命运

• 批准号: 1266243
• 负责人: Elise Morgan
• 金额: $ 28.01万
• 依托单位: Trustees of Boston University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-01 至 2017-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1266243&HistoricalAwards=false
• 关键词: Tracking; Cell; Fate; during; Mechanically; Tracking; Cell; Fate; during; Mechanically

The research objective of this award is to quantify the mechanical environment of a bone injury and its association with the response of skeletal progenitor cells at the injury site. Skeletal progenitor cells play a principal role in healing of bone fractures and other skeletal injuries and can differentiate into one of multiple, committed cell types depending on stimuli in their local environment. The planned studies will apply a combination of experimental and computational tools from engineering and biology to label progenitor cells at the injury site, to quantify the mechanical environment of the tissues in which these cells reside, and to identify the specific mechanical stimuli (e.g., shear strain, pressure, etc.) associated with the recruitment of these cells and their various fates. These studies will define the mechanical conditions highly favorable to formation of a given type of skeletal tissue, such as bone, cartilage, and fibrous tissue.These studies will accelerate the pace of discovery in several areas of basic and clinical research, including defining mechanical environments that can promote bone healing, uncovering the mechanisms by which mechanical stimuli can influence cell differentiation, and identifying new classes of molecular targets for enhancing bone formation. Each of these avenues is directed towards alleviating the personal and economic costs arising from bone injuries, and in particular, from poor healing of these injuries. The educational components of the project consist of training of junior scientists and engineers-two graduate students and three undergraduate students will be mentored on this research project-and on enhancing a successful, residential summer program for low-income, high-school girls interested in scientific and quantitative fields of study. Content for the program is supplied by university faculty and staff, industry partnerships, and outreach modules designed by undergraduate and graduate students enrolled in courses in engineering mechanics and biomechanics.

该奖项的研究目标是量化骨损伤的机械环境及其与骨骼祖细胞在损伤部位的反应的关联。骨骼祖细胞在骨骼骨折和其他骨骼损伤的愈合中起主要作用，并且可以根据其当地环境中的刺激分化为多种，承诺的细胞类型之一。计划的研究将采用从工程和生物学到损伤部位标记祖细胞的实验和计算工具的组合，量化与这些细胞所在的组织的机械环境，并识别与这些细胞及其各种命运相关的特定机械刺激（例如，剪切应变，压力等）。这些研究将定义高度有利于形成给定类型的骨骼组织（例如骨骼，软骨和纤维组织）的机械条件。这些研究将加速基本和临床研究的几个领域的发现速度，包括定义可以促进骨骼的骨骼刺激的机械性刺激的机制，并鉴定出骨骼的差异，并确定了骨骼的差异。这些途径中的每一个都用于减轻骨骼损伤所产生的个人和经济成本，尤其是由于这些伤害的治愈不佳而引起的。该项目的教育组成部分包括对初级科学家和工程师的培训，两位研究生和三名本科生将受到这项研究项目的指导，并针对对科学和定量研究领域感兴趣的低收入，高中女孩的成功住宅夏季计划。该计划的内容由大学教职员工，行业合作伙伴关系以及由本科生和研究生设计的外展模块提供，并参加了工程机械师和生物力学课程。