Low-Power Stimulated Raman Time-Lapse Microscope for Tracking Dynamics and Fate of Lipid Droplets in Glioma Cells

低功率受激拉曼延时显微镜用于跟踪神经胶质瘤细胞中脂滴的动态和命运

• 批准号: 10114793
• 负责人: Fake Lu
• 金额: $ 43.33万
• 依托单位: STATE UNIVERSITY OF NY,BINGHAMTON
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-24 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10114793
• 关键词: Acid Lipase; Adopted; Area; Axon; Behavior; Biophotonics; Brain; Cell Proliferation; Cell Survival; Cells; Clinical; Data; Detection; Emerging Technologies; Environment; Fatty Acids; Fiber; Fluorescence; Fresh Tissue; Generations; Glioblastoma; Glioma; Goals; Health Sciences; Human; Hydrolysis; Hypoxia; Image; Image-Guided Surgery; Imaging technology; Infiltration; Label; Lasers; Legal patent; Life Cycle Stages; Light; Lipids; Lysosomes; Malignant Neoplasms; Microscope; Microscopy; Monitor; Mus; Myelin Sheath; Nutrient; Organelles; Pattern; Pharmacologic Substance; Physiological; Play; Process; Pump; Research; Role; Scheme; Signal Pathway; Signal Transduction; Silicon; Slice; Source; Specimen; Starvation; Stress; Structure; Supervision; Therapeutic Intervention; Time; Tissues; Universities; anti-cancer therapeutic; brain tissue; cancer cell; cancer survival; cancer type; chemical bond; detector; doctoral student; flexibility; hands on research; imaging platform; imaging system; improved; lipid biosynthesis; lipid metabolism; live cell imaging; migration; new technology; novel; nutrient deprivation; therapeutic target; time use; tool; tumor; tumor microenvironment; two-photon; undergraduate student; vibration

Project Summary/Abstract Lipid droplets (LDs) is highly dynamic cellular organelles that play multiple functions in cell lipid metabolism. Reprogramming of lipid metabolism is now considered a hallmark of cancer. LDs accumulate within the cancer cells due to multiple reasons, but the mechanism has not been fully understood. In this project, we will develop new technologies to image the dynamics of LDs in cancer cells. Stimulated Raman scattering (SRS) microscopy is a powerful tool to image label-free LDs in live cells and tissue. However, the laser power used in SRS imaging is too high to conduct long-term time-lapse imaging of live cells. The objective of this AREA project is to establish a low-laser-power time-lapse stimulated Raman microscope and use the imaging platform to depict the dynamics and fate of label-free LDs for understanding their function in glioma progression and their role as a potential therapeutic target. Specific aims include tracking the dynamics and life cycle of lipid droplets formation and degradation in glioma cells and tracking migration and invasion of glioma cells in cultured organotypic mouse brain tissue. The results of this project will advance our understanding on the condition-specific roles of LDs in cancer survival and aggressiveness and will provide new clues to target LDs for potential therapeutic intervention. This AREA project would expose one PhD student and nine undergraduate students to hands on research under the PI’s supervision. Through conducting this project, the research environment at the Binghamton University will be beneficially enhanced in the field of health science and biophotonics.

项目摘要/摘要 脂质液滴（LDS）是高度动态的细胞细胞器，在细胞脂质代谢中起多种功能。 重新编程脂质代谢现在被认为是癌症的标志。 LD在癌症中积累 细胞由于多种原因，但尚未完全了解该机制。在这个项目中，我们将开发 新技术以图像癌细胞中LDS的动力学。刺激的拉曼散射（SRS）显微镜 是在活细胞和组织中图像无标签LDS的强大工具。但是，SRS成像中使用的激光功率 太高了，无法对活细胞进行长期延时成像。该区域项目的目的是建立 低激光驱动的延时刺激的拉曼显微镜，并使用成像平台描述动力学 和无标签LD的命运，以了解其在神经胶质瘤进展中的功能及其作为潜力的作用 治疗靶标。具体目的包括跟踪脂质液滴形成的动态和生命周期和生命周期 神经胶质瘤细胞中的降解以及跟踪培养的有机小鼠中神经胶质瘤细胞的迁移和侵袭 脑组织。该项目的结果将提高我们对LDS中特定条件特定角色的理解 癌症的生存和侵略性，将为可能的LDS提供新的线索，以进行潜在的治疗干预。 该地区项目将使一名博士生和九名本科生在研究下进行研究 PI的监督。通过进行该项目，宾厄姆顿大学的研究环境 在健康科学和生物探测领域，将有益地增强。