Research Starter Grant: Functional analysis of the Arabidopsis heterotrimer G-protein beta subunit, AGB1 and putative AGB1-interacting proteins in mediating stress responses

研究启动资助：拟南芥异源三聚体 G 蛋白 β 亚基、AGB1 和假定的 AGB1 相互作用蛋白在介导应激反应中的功能分析

• 批准号: 1237301
• 负责人: Tyrell Carr
• 金额: $ 5万
• 依托单位: Chowan University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-15 至 2014-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1237301&HistoricalAwards=false
• 关键词: Research; Starter; Grant; Functional; analysis

Functional analysis of the Arabidopsis heterotrimer G-protein beta subunit, AGB1 and putative AGB1-interacting proteins in mediating stress responsesIn response to environmental stress, plant growth, development and/or yield can be adversely affected. Recently, the Arabidopsis thaliana heterotrimer G-protein beta subunit (AGB1) has been linked to Arabidopsis seed production in response to water availability and an AGB1-interacting protein has been linked to Arabidopsis susceptibility to a bacterial pathogen. With the release of the Arabidopsis G-protein interactome, it is greatly anticipated that the role of AGB1 in abiotic stress and biotic stress responses will be determined, since it is largely unknown. The funded project will examine the role of putative AGB1-interacting proteins toward plant fitness in response to drought conditions and in response to bacterial and viral infections. Using various Arabidopsis genetic backgrounds, a functional genetics approach coupled with biochemical and molecular approaches will be used to detect altered hormonal and signaling pathways in response to stress stimuli. The intellectual merit of this proposal consists of the following: (1) deciphering the Arabidopsis AGB1 signaling pathway in response to drought/abiotic stress and pathogen/biotic stress and (2) identifying the functional roles of putative Arabidopsis AGB1-interacting proteins in mediating these responses. The broader scope of this research includes furthering plant science initiatives to increase the yield of essential plant products for energy applications, agriculture, forestry and other beneficial needs. The funded project will provide numerous research opportunities to increase the number of students including minorities and women from Chowan University and surrounding communities that enter science, technology, engineering and mathematics (STEM) careers including the plant sciences. In addition, the funded project will help strengthen the STEM curriculum at Chowan University and further undergraduate student and local middle school/high school student advancement in interdisciplinary learning. Chowan University is a small primarily undergraduate institution with a growing minority enrollment located in a rural and underdeveloped section of northeastern North Carolina.

拟南芥异源三聚体G蛋白β亚基、AGB1和推定的AGB1相互作用蛋白在介导胁迫反应中的功能分析响应环境胁迫，植物生长、发育和/或产量可能受到不利影响。最近，拟南芥异源三聚体 G 蛋白 β 亚基 (AGB1) 与拟南芥种子生产响应水可用性有关，并且 AGB1 相互作用蛋白与拟南芥对细菌病原体的易感性有关。随着拟南芥G蛋白相互作用组的发布，人们非常期待AGB1在非生物胁迫和生物胁迫反应中的作用将被确定，因为它在很大程度上是未知的。该资助项目将研究假定的 AGB1 相互作用蛋白在植物适应干旱条件以及细菌和病毒感染方面的作用。利用各种拟南芥遗传背景，功能遗传学方法与生化和分子方法相结合，将用于检测响应应激刺激而改变的激素和信号传导途径。该提案的智力价值包括以下内容：(1) 破译拟南芥 AGB1 信号通路响应干旱/非生物胁迫和病原体/生物胁迫的作用；(2) 确定假定的拟南芥 AGB1 相互作用蛋白在介导这些胁迫中的功能作用。回应。这项研究的更广泛范围包括进一步推进植物科学举措，以提高能源应用、农业、林业和其他有益需求的基本植物产品的产量。该资助项目将提供大量研究机会，以增加乔万大学及周边社区的少数民族和女性学生数量，进入包括植物科学在内的科学、技术、工程和数学 (STEM) 职业。此外，资助项目将有助于加强乔文大学的STEM课程，并进一步促进本科生和当地中学生/高中生在跨学科学习方面的进步。乔万大学是一所小型本科院校，位于北卡罗来纳州东北部的农村和欠发达地区，少数族裔入学人数不断增加。