I-Corps: High Frame-rate Fluorescence Lifetime Imaging Microscopy

I-Corps：高帧率荧光寿命成像显微镜

• 批准号: 1243899
• 负责人: Kenneth Shepard
• 金额: $ 5万
• 依托单位: Columbia University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1243899&HistoricalAwards=false
• 关键词: Corps; Frame; rate; Fluorescence; Lifetime

The High Frame Rate Fluorescence Lifetime Imaging Microscopy system promises new fluorescence imaging capabilities at costs dramatically lower than current systems. By integrating detectors and converters on a single platform, frame rates in excess of 100 frames/second at a production cost of under $1000 should be possible. This compares with image acquisition times of a few seconds per frame at near 100 times the cost fopr currently-available systems. This proposal will assist the team in assessing the commercial viability of the research that was previously sponsored by NSF. If successfully commercialized, the team will deliver a new technology that advances the state of the art in fluorescence lifetime imaging microscopy. This advancement will allow measurements of dynamics of fluorescence lifetimes with an inexpensive and simple system. These measurements could lead to further advances in microscopy as well as the development of new medical diagnostic systems. In addition to the scientific advances that would be possible with widespread adoption of the system, there would also be positive economic impacts that would result from transitioning the hardware from the lab and ramping production.

高帧速率荧光寿命成像显微镜系统承诺，成本大大低于当前系统的成本大大低于成本。通过将检测器和转换器集成在一个平台上，应以低于1000美元的生产成本超过100帧/秒的帧速率。相比之下，图像采集时间为每帧几秒钟，其成本是当前可用系统的100倍。该提案将有助于该团队评估NSF先前赞助的研究的商业可行性。如果成功商业化，该团队将提供一项新技术，以荧光寿命成像显微镜在荧光寿命成像中发展。这种进步将允许使用廉价且简单的系统来测量荧光寿命的动力学。这些测量可能会导致显微镜以及新的医学诊断系统的发展进一步进步。除了广泛采用该系统的科学进步外，还将产生积极的经济影响，这将导致从实验室过渡和逐步生产的硬件。