MRI: Acquisition of an Illumina HiSeq2000 as a core sequencing instrument for genomics and gene expression research

MRI：购买 Illumina HiSeq2000 作为基因组学和基因表达研究的核心测序仪器

• 批准号: 1229046
• 负责人: Michael Axtell
• 金额: $ 56.94万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-10-01 至 2015-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1229046&HistoricalAwards=false
• 关键词: MRI; Acquisition; Illumina; HiSeq2000; core; MRI; Acquisition; Illumina; HiSeq2000; core

This Major Research Instrumentation award supports the purchase of an Illumina HiSeq2500 DNA sequencing instrument for the Genomics Core Facility at Penn State's University Park campus. Sequencing of DNA is a key component of modern life sciences research. In the last decade, technologies for DNA sequencing have advanced at an astonishing rate. In that time frame, data output per instrument "run" has increased by more than a trillion-fold. Consequently, the amount of DNA information available has skyrocketed, enabling revolutionary advances across all of the biosciences. The Illumina HiSeq2500 is the most powerful sequencing instrument available to date, both in terms of data output per unit time, and cost per DNA-base sequenced. Its very high quality, relatively long read lengths, and well-established sequencing chemistry allow the maximum experimental flexibility. It is capable of generating DNA sequence data to allow de novo genome assemblies, as well as re-sequencing and transcriptome studies. Installation of this instrument into the Genomics Facility will significantly enhance life science research at Penn State and beyond, particularly in three broad areas: Genomics and gene expression of non-model organisms, genomics of multispecies interactions, and regulation of gene expression. Given the flexibility and robust performance of this instrument, it is likely that many additional, as yet unforeseen areas of study will be enhanced by this instrument.This instrument's capabilities will dramatically enhance life sciences research at Penn State. In addition, it will enhance interdisciplinary scientific training for Penn State researchers at the undergraduate, graduate, and post-doctoral levels. It's use and data will be integrated into existing hands-on graduate training programs and courses, and used as part of the regular series of workshops offered by the Genomics Core Facility. Access and training will be provided, through several existing programs, to several populations of students who are currently under-represented in the scientific enterprise. Experiments conducted on this instrument will also be used to enhance Penn State's continuing involvement with a consortium of predominantly undergraduate institutions for scientific training.

这项重大的研究仪器奖支持购买宾夕法尼亚州立大学公园校园基因组核心设施的Illumina HISEQ2500 DNA测序工具。 DNA的测序是现代生命科学研究的关键组成部分。 在过去的十年中，DNA测序的技术以惊人的速度提高了。 在那个时间范围内，每个仪器“运行”的数据输出增加了超过万亿倍。 因此，可用的DNA信息量飙升，从而在所有生物科学中都能革命性进步。 Illumina HISEQ2500是迄今为止可用的最强大的测序仪器，无论是每单位时间的数据输出而言，每个DNA碱基的成本是测序的。 它的高质量，相对较长的读取长度和完善的测序化学允许最大的实验灵活性。 它能够生成DNA序列数据以允许从头基因组组件以及重新测序和转录组研究。 将该仪器安装到基因组学设施中将显着增强宾夕法尼亚州及其他地区的生命科学研究，尤其是在三个广泛的领域：非模型生物的基因组学和基因表达，多品种相互作用的基因组学以及基因表达的调节。 鉴于该乐器的灵活性和强大的性能，该工具可能会增强许多其他研究领域的其他研究领域。本文的能力将大大增强宾夕法尼亚州立大学的生命科学研究。 此外，它将增强本科，研究生和博士后水平的宾夕法尼亚州立大学研究人员的跨学科科学培训。 它的使用和数据将集成到现有的动手研究生培训计划和课程中，并用作基因组核心设施提供的常规研讨会的一部分。 通过几个现有计划，将向几个目前在科学企业中代表不足的学生提供访问和培训。 在该工具上进行的实验还将用于增强宾夕法尼亚州立大学的持续参与，主要是本科机构进行科学培训。