MRI: Acquisition of an Illumina NovaSeq 6000 to Support High-Throughput Sequencing Collaborative Research and Integrated Training

MRI：采购 Illumina NovaSeq 6000 以支持高通量测序协作研究和综合培训

• 批准号: 2117272
• 负责人: Frank Loeffler
• 金额: $ 69.25万
• 依托单位: University of Tennessee Knoxville
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-10-01 至 2024-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2117272&HistoricalAwards=false
• 关键词: MRI; Acquisition; Illumina; NovaSeq; 6000; MRI; Acquisition; Illumina; NovaSeq; 6000

An award is made to The University of Tennessee, Knoxville (UTK) to acquire an Illumina NovaSeq sequencer, which will be broadly accessible to on-campus and external researchers through the Genomics Core within the Center for Environmental Biotechnology. The new instrumentation will enable UTK researchers to attain a level of competency more attuned to the breadth of research across the institution’s portfolio of microbial, plant, human, and animal nucleic acid sequencing needs. The expanded Genomics Core will engage a broader cohort of researchers to obtain sequence data, provide faculty new means of illustration of the genes-to-ecosystem concept, and excite more young minds about discovery-driven scientific research and the exploration of the rules of life. A robust network of outreach activities that align with NSF’s STEM educational mission will be strategically integrated into new training and learning opportunities involving the NovaSeq. The newly enabled research will address fundamental research questions that impact the applied sciences and engineering disciplines, and several projects will be of considerable public interest. The NovaSeq sequencing instrument bestows the ability to perform demanding nucleic acid sequencing projects allowing researchers to assess the entire genetic makeup of a single organism (e.g., a bacterium, a fungus, or an animal), or an assembly of different microorganisms in a specific habitat (i.e., a microbiome). DNA sequencing will reveal microbial taxonomy and functional potential, and metatranscriptome sequence data will shed light on the actual activities that result in observable characteristics (i.e., organismal phenotypes). Research projects will link microbiome sequence information with processes relevant to environmental, animal, and human health. Sequence-enabled research projects will advance understanding of microbes that detoxify pollutants, control greenhouse gas emission, and promote carbon dioxide sequestration in (agricultural) soils; generate new opportunities for ecologically informed control of biocorrosion and biofouling; allow integrated cross-kingdom analyses to reveal host-microbiome interactions and genetic and epigenetic mechanisms that determine phenotypes; compare lives of contemporary and ancient peoples; explore microbiomes in understudied environments, such as the deep subsurface; and provide new insights into viral ecology and emerging viral diseases (i.e., zoonoses).This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

授予田纳西大学诺克斯维尔大学（UTK）的奖励，以获取Illumina Novaseq Sequencer，校园和外部研究人员将通过环境生物技术中心内的基因组学核心广泛访问。新的仪器将使UTK研究人员能够在该机构的微生物，植物，人类和动物核酸测序需求中更加满足一定程度的能力。扩展的基因组学核心将参与更广泛的研究人员，以获取序列数据，为基因到生态系统概念提供新的说明方法，并激发更多有关发现驱动的科学研究的年轻人和对生活规则的探索。与NSF的STEM教育任务保持一致的强大外展活动网络将在战略上融入涉及Novaseq的新培训和学习机会中。新启用的研究将解决影响应用科学和工程学科的基本研究问题，几个项目将引起公共利益。 NovaseQ测序仪器具有执行要求核酸测序项目的能力，使研究人员能够评估单个生物体（例如细菌，真菌或动物）的整个遗传组成，或者在特定生t（即Microbiome）中组装不同的微生物。 DNA测序将揭示微生物分类学和功能潜力，元转录组序列数据将阐明导致可观察到特征的实际活动（即有机表型）。研究项目将将微生物组序列信息与与环境，动物和人类健康有关的过程联系起来。支持序列的研究项目将提高对污染物，控制温室气体排放并促进（农业）土壤中二氧化碳会议的微生物的了解；为生态知情的生物腐蚀和生物污染带来新的机会；允许综合的跨王朝分析揭示确定表型的宿主 - 微生物组相互作用以及遗传和表观遗传机制。比较当代和古代人的生活；在可理解的环境中探索微生物组，例如深度地面；并提供有关病毒生态学和新兴病毒疾病的新见解（即人畜共患病）。该奖项反映了NSF的法定使命，并使用基金会的知识分子优点和更广泛的影响来评估，被认为是宝贵的支持。