OCE-PRF: Carbon dioxide, pH and bicarbonate sensing pathways in reef-building corals and regulation of homeostasis in response to ocean acidification

OCE-PRF：造礁珊瑚中的二氧化碳、pH 值和碳酸氢盐传感途径以及响应海洋酸化的稳态调节

• 批准号: 1226396
• 负责人: Katie Barott
• 金额: $ 17万
• 依托单位: Barott Katie L
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2014-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1226396&HistoricalAwards=false
• 关键词: OCE; PRF; Carbon; dioxide; pH

The investigator will study the cellular mechanisms of how reef-building corals sense changes in pH in the surrounding seawater and how the regulation of this pathway affects coral physiological responses to ocean acidification (OA). This project builds on the recent discovery that a bicarbonate (HCO3-) sensing enzyme (soluble adenylyl cyclase [sAC]) involved in the regulation of pH homeostasis in vertebrates is expressed in corals. This enzyme produces cAMP, a universal messenger molecule used in organisms from bacteria to mammals. The investigator's hypothesis is that the sAC pathway is involved in coral pH sensing and regulation of pH homeostasis through the production of cAMP. Coral sAC is stimulated in response to HCO3- concentrations relevant to coral physiology, and differences in sAC activity and regulation will affect coral physiological responses to OA. To test her hypothesis, the investigator will: 1. Characterize the activity and expression of the sAC pathway and its response to CO2, HCO3- and pH in multiple coral species, 2. Determine how the sAC pathway affects coral physiology, including maintenance of pH homeostasis, respiration, photosynthesis, and calcification, 3. Determine how chronic exposure to projected OA conditions affect the regulation of the sAC pathway in corals and how these differences affect coral physiology. The broader impacts of the project include developing a bilingual, multimedia exhibit showcasing the significance of ocean acidification and its effects on the marine environment at the Birch Aquarium in San Diego, reaching a large Latino population, and mentoring two female doctoral students and undergraduates. This project is supported under the NSF Ocean Sciences Postdoctoral Research Fellowship (OCE PRF) program, with goals to support novel research by early career scientists and increase the diversity of the U.S. ocean sciences workforce and research community. With OCE-PRF support, this project will enable a promising early career researcher to establish themselves in an independent research career related to ocean sciences and broaden participation of under-represented groups in the ocean sciences.

研究者将研究珊瑚礁珊瑚如何感觉周围海水中pH的变化以及该途径的调节如何影响珊瑚生理对海洋酸化（OA）的调控。该项目建立在最近发现的基础上，即碳酸氢盐（HCO3-）传感酶（可溶性腺苷酸环化酶[SAC]）参与脊椎动物中pH稳态调节的调节。这种酶产生cAMP，这是一种通用的信剂分子，用于从细菌到哺乳动物的生物体。研究者的假设是SAC途径通过营地的生产参与珊瑚pH感应和pH稳态的调节。响应于与珊瑚生理有关的HCO3浓度刺激珊瑚囊，而SAC活性和调节的差异将影响珊瑚生理反应对OA。为了检验她的假设，研究者将：1。表征SAC途径的活性和表达及其对多种珊瑚物种中的CO2，HCO3和pH的反应，2。确定囊途径如何影响珊瑚生理学，包括维持pH稳定性，包括呼吸稳定性，呼吸，光合作用，光合作用和钙化的范围的partectution partosition Shecruation of Shitection of Project of Project of Partioned Oaa的partions partivation Shemai Aa oaa partions partioned oaa oaa的范围。影响珊瑚生理。该项目的更广泛影响包括开发双语的多媒体展示了海洋酸化的重要性及其对圣地亚哥桦木水族馆海洋环境的影响，并获得了大量拉丁裔人口，并指导了两名女性博士生和培训。该项目得到了NSF海洋科学博士后研究奖学金（OCE PRF）计划的支持，其目标是支持早期职业科学家的新研究，并增加了美国海洋科学工作人员和研究社区的多样性。在OCE-PRF的支持下，该项目将使有前途的早期职业研究人员能够在与海洋科学有关的独立研究职业中建立自己，并扩大了代表性不足的群体在海洋科学中的参与。