U.S.-Netherlands Planning Visit: Binding and Recognition of Complex Glycolipids by Mycobacterial Proteins

美国-荷兰计划访问：分枝杆菌蛋白对复杂糖脂的结合和识别

• 批准号: 1157639
• 负责人: Jessica Seeliger
• 金额: $ 1.36万
• 依托单位: SUNY at Stony Brook
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-10-01 至 2013-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1157639&HistoricalAwards=false
• 关键词: U.S.; Netherlands; Planning; Visit; Binding

Through this planning visit, researcher Jessica Seeliger of SUNY at Stony Brook will initiate a cooperative project with partners in the Netherlands at the University of Groningen. Her counterpart, Adriaan Minnaard, and his group have established expertise in total synthesis of mycobacterial lipids and their analogues, which complements Seeliger's studies of enzymatic mechanistic and substrate activity. Together, their intent is to investigate the protein machinery and catalytic mechanisms behind lipid biosynthesis and transport in mycobacteria. Their focus on chemical determinants of molecular recognition should improve our knowledge of how hydrophobic substrates are recognized for transport from the cytosol, where they are made, to the outer membrane where they reside. Results from the planned long-term collaboration are expected to improve our fundamental understanding of lipid biogenesis in mycobacteria. If successful, the researchers expect to gain new information about chemical probes that would be useful not only to the mycobacterial research community, but also to others studying lipids in other bacteria. This catalytic research visit fulfills the program objective of advancing basic biological knowledge by enabling experts in the United States and Europe to share research resources and plan beneficial projects in areas of strong mutual interest and competence. The experience gained through examination of biosynthetic pathways and transport of unusual lipids, particularly in Actinobacteria, may have broader impact by contributing to our ability to identify immunological receptors for bacterial antigens. Furthermore, one participating U.S. graduate student will gain a valuable early career introduction to international collaborative teamwork.

通过这次计划访问，Stony Brook的Suny的研究员Jessica Seeliger将与荷兰的Groningen大学的合作伙伴进行合作项目。 她的对手Adriaan Minnaard及其小组在整体合成分枝杆菌脂质及其类似物方面建立了专业知识，这补充了Seeliger对酶促机械和底物活性的研究。他们的目的共同研究了分枝杆菌中脂质生物合成和转运背后的蛋白质机制和催化机制。 他们对分子识别的化学决定因素的关注应提高我们对疏水性底物的认识，从而识别出从制造的细胞质转运到居住的外膜的运输。 预计计划中的长期合作的结果将改善我们对分枝杆菌中脂质生物发生的基本理解。 如果成功的话，研究人员希望获得有关化学探针的新信息，这些信息不仅对分枝杆菌研究界有用，还可以对其他细菌中脂质的其他信息有用。 这项催化研究的访问实现了计划的目标，即通过使美国和欧洲的专家能够共享研究资源，并计划在浓厚的共同利益和能力领域中计划有益的项目，从而提高基本生物学知识。 通过检查生物合成途径和不寻常脂质的运输，特别是在静脉细菌中获得的经验，可能会通过促进我们鉴定细菌抗原免疫学受体的能力而产生更大的影响。 此外，一位参加美国研究生将获得国际合作团队合作的宝贵早期职业介绍。