Fundamental and Mechanistic Studies of Negative Ion Electron Capture Dissociation (niECD)

负离子电子捕获解离（niECD）的基础和机理研究

• 批准号: 1152531
• 负责人: Kristina Hakansson
• 金额: $ 40万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-04-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1152531&HistoricalAwards=false
• 关键词: Fundamental; Mechanistic; Studies; Negative; Ion

This award by the Chemical Measurement and Imaging (CMI) program supports work by Professor Kristina Håkansson at the University of Michigan to explore the mechanism and improve the analytical utility of negative ion electron capture dissociation (niECD), a novel tandem mass spectrometry technique recently discovered in the PI's laboratory. The goal of this research is to further examine the counter-intuitive process of niECD, which results in charge-increased rather than the typical charge-decreased species in other ion-electron reactions. Further development of niECD will allow access to novel means for detailed structural characterization of important low-level acidic biomacromolecules such as phospho- and sulfopeptides, intact acidic proteins, and RNA-small molecule complexes. The educational goals of this work include training of graduate students in an interdisciplinary environment as well as participation in a K-12 Summer Program "Girls in Science and Engineering" offered by the University of Michigan's Women in Science and Engineering Program. The latter activities are aimed at boosting an interest in the scientific method at a young age through the discovery that the contents of a box can be elucidated without opening it. The broader impact of the research includes introduction of novel technology for biomolecular structural characterization with important implications for drug discovery and an enhanced understanding of the molecular basis of living organisms.

化学测量和成像（CMI）计划的这一奖项支持密歇根大学的KristinaHåkansson教授的工作，以探索该机制并改善负离子电子捕获解离的分析效用（NIECD），这是一种新型的串联量质谱技术，最近在PI的实验室中发现了。这项研究的目的是进一步检查NIECD的违反直觉过程，该过程导致电荷增加，而不是其他离子电子反应中典型的电荷被束缚的物种。 NIECD的进一步发展将允许获得新的手段，以详细的结构表征对重要的低水平酸性生物乳清分子，例如磷酸和硫肽，完整的酸性蛋白质以及RNA-SMALL分子络合物。这项工作的教育目标包括在跨学科环境中培训研究生，以及参与密歇根大学科学与工程计划女性提供的K-12夏季课程“科学与工程女孩”。后者的活动旨在通过发现可以阐明盒子的内容而不打开它，从而增强对科学方法的兴趣。这项研究的更广泛的影响包括引入新型的生物分子结构表征的技术，对药物发现的重要意义以及对生物体分子基础的增强理解。