RUI: Cell Wall Associated Protein Kinases As Pectin Receptors

RUI：细胞壁相关蛋白激酶作为果胶受体

• 批准号: 1146245
• 负责人: Bruce Kohorn
• 金额: $ 40万
• 依托单位: Bowdoin College
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2016-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1146245&HistoricalAwards=false
• 关键词: RUI; Cell; Wall; Associated; Protein

All organisms are made up of collections of cells, or bags of material that each carry out essential processes for life. Each cell has a program that can be influenced by the environment to enlarge, divide, and form specific shapes. The ultimate shape, size and function of a plant is therefore determined by the nature of each individual cell. Plant cells are surrounded by structurally strong but plastic material called the cell wall, that includes the carbohydrates cellulose and pectin. The cell wall has a direct influence on the size and shape that each cell reaches as it acts as a cage around each cell, and hence modification of the cell wall has a direct affect on cell shape and size. The cell surface contains a protein molecule, called a wall-associated kinase (WAK), that binds to the cell wall pectin and senses the structure and relays this to the inside of the cell to cause changes in growth and responses to the environment. Indeed, pathogens and wounding directly affect pectins, and WAKs aid in the sensing of this damage. WAKs are also required for normal plant growth. This project will concentrate on the mechanism by which WAKs bind and sense pectin changes so as to play a dual role differentiating growth from the stress response. Genetic and biochemical analysis using the model plant Arabidopsis will generate paradigms likely applicable to all types of flowering plants. Undergraduate students involved in the work will be provided with an integrative approach to science, and will be prepared for the challenges of graduate and professional schools and the private sector upon graduation.

所有生物都由细胞的集合或每种材料袋组成的材料组成。 每个单元都有一个程序，可以受到环境的影响，以扩大，分裂和形成特定形状。因此，植物的最终形状，大小和功能由每个单个细胞的性质决定。植物细胞被结构强但称为细胞壁的塑料包围，其中包括碳水化合物纤维素和果胶。细胞壁对每个细胞的大小和形状都有直接影响，因为它充当每个细胞周围的笼子，因此细胞壁的修饰对细胞形状和大小都有直接影响。 细胞表面包含一个蛋白质分子，称为壁相关激酶（WAK），该蛋白质与细胞壁果胶结合并感应结构，并将其转移到细胞的内部，以引起生长和对环境的反应的变化。 实际上，病原体和伤口直接影响果胶，并挥动有助于感知这种损害。 正常的植物生长也需要WAKS。该项目将集中于waks结合和感觉果胶变化的机制，从而发挥双重作用，将生长与压力反应区分开。使用模型植物拟南芥的遗传和生化分析将产生可能适用于所有类型的开花植物的范例。参与这项工作的本科生将采用一种综合科学方法，并在毕业后为研究生和专业学校以及私营部门的挑战做好准备。