RUI: Cell Wall Associated Protein Kinases

RUI：细胞壁相关蛋白激酶

• 批准号: 0543290
• 负责人: Bruce Kohorn
• 金额: $ 7.62万
• 依托单位: Bowdoin College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-15 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0543290&HistoricalAwards=false
• 关键词: RUI; Cell; Wall; Associated; Protein

The shape and size of a plant cell can be determined by a variety of genetic and environmental factors, and involves an interplay between cell turgor and a regulated loosening of the surrounding cell wall or extracellular matrix. The regulation of sugar metabolism, uptake and transport also plays a central role in these growth processes. This project involves Arabidopsis receptor like protein kinases, the cell Wall Associated Kinases (WAKs), that are linked to pectin and are required for cell expansion. WAK attachment to pectin in the cell wall may be part of a mechanism that leads to the regulation of vacuolar invertase, and this in turn could influence turgor and hence cell expansion. Mutations in WAKs lead to a loss of cell expansion and in a wak2-1 mutant allele, a reduction of invertase activity in the vacuole. There are five WAKs in the Arabidopsis genome, and it has already been established that WAK2 is required for cell elongation and solute metabolism. The experiments will explore whether two other WAKs, WAK1 and WAK4 which are responsible for most of the remaining WAK activity in Arabidopsis, play the same role. The phenotypes of mutations in WAK1 and 4 will be explored by determining whether, like wak2-1, both sugars and salts are required for growth. A microscopic analysis will be used to determine if the mutations affect cell elongation. Enzyme activity and mRNA levels for key enzymes regulating solute metabolism will be measured to determine if the wak1 and wak4 alleles have the same affect as does the wak2-1 mutation. Thus it will be established whether other WAK family members have similar or distinct roles in cell expansion and plant development. These studies will explore fundamental events in cells that determine their shape and size, and thus architecture and function of plants. The research will be carried out at small liberal arts college, where undergraduate students will be provided with an integrative approach to science, and be prepared for the challenges of graduate and professional schools upon graduation.

植物细胞的形状和大小可以通过多种遗传和环境因素来确定，涉及细胞爆炸与周围细胞壁或细胞外基质的调节松动之间的相互作用。糖代谢，摄取和运输的调节在这些生长过程中也起着核心作用。 该项目涉及拟南芥受体，例如蛋白激酶，与细胞壁相关激酶（WAKS），它们与果胶有关，是细胞膨胀所必需的。细胞壁中果胶对果胶的附着可能是导致液泡转化酶调节的机制的一部分，这反过来又会影响爆炸，从而影响细胞的膨胀。 WAKS中的突变导致细胞膨胀和WAK2-1突变等位基因的丧失，液泡中转化酶活性的降低。拟南芥基因组中有五个WAK，已经确定WAK2是细胞伸长和溶质代谢所必需的。该实验将探讨其他两个WAKS，WAK1和WAK4是否负责拟南芥中剩余的WAK活动，它们是否扮演相同的角色。 WAK1和4中突变的表型将通过确定像WAK2-1一样，生长所需的糖和盐。微观分析将用于确定突变是否影响细胞伸长。将测量调节溶质代谢的关键酶的酶活性和mRNA水平，以确定WAK1和WAK4等位基因是否具有与WAK2-1突变相同的影响。因此，将确定其他WAK家族成员在细胞扩张和植物发育中具有相似或独特的作用。 这些研究将探索细胞中确定其形状和大小的基本事件，从而确定植物的结构和功能。 这项研究将在小型文科学院进行，在该学院为本科生提供一种综合的科学方法，并为毕业后的研究生和专业学校的挑战做好准备。