Meeting on the Future of Plant Genome Sequencing and Analysis to be held May 18-20, 2011 at Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

关于植物基因组测序和分析的未来的会议将于 2011 年 5 月 18 日至 20 日在纽约冷泉港冷泉港实验室举行。

• 批准号: 1135736
• 负责人: William McCombie
• 金额: $ 4.88万
• 依托单位: Cold Spring Harbor Laboratory
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-05-01 至 2012-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1135736&HistoricalAwards=false
• 关键词: Meeting; Future; Plant; Genome; Sequencing

The last few years have seen a revolution in DNA sequencing instrumentation and technology. In that short time period sequencing instrument capabilities have increased more than 1000 fold and are likely to continue to increase about 5-fold each year for the next several years. As such it is now affordable and efficient to sequence to high coverage large plant genomes that had previously been prohibitively too expensive and complex to attempt. However, analysis methods have not improved nearly as much during the same time period and a variety of technical limitations of these new DNA sequencing instruments make it even more difficult to carry out whole genome sequencing of novel genomes (de novo sequencing). These limitations also make it more difficult to use the new instruments to carry out older clone based strategies for de novo sequencing, such as BAC-by-BAC approaches. The purpose of this meeting to be held at Cold Spring Harbor Laboratory May 18-20, 2011 are to assess the current state of next generation sequencing in terms of de novo, whole genome plant sequencing, what can be expected to develop in the near future, and then determine which advances are needed to allow these exciting technologies to be used to carry out de novo sequencing of entire complex plant genomes. The meeting will bring together stakeholders with broad range of expertise in high-throughput sequencing and genomics, plant biology, bioinformatics and databases drawn from the academic, private and international sectors. Meeting outcomes will be captured in the form of a report to be developed by participants that will be submitted for publication to Genome Research.

在过去的几年中，DNA测序仪器和技术发生了革命。 在短时间内，测序仪器能力增加了1000倍以上，并且在接下来的几年中，每年可能会继续增加约5倍。因此，现在可以负担得起且有效地将高覆盖范围的大型植物基因组顺序排序，这些基因组先前过于昂贵且复杂而无法尝试。但是，在同一时期，分析方法并没有得到太大的改善，这些新的DNA测序仪器的各种技术局限性使得对新基因组进行整个基因组测序（从头测序）变得更加困难。这些局限性还使使用新工具来执行基于克隆的较旧的策略进行从头测序，例如BAC-BAC方法。这次会议的目的是在2011年5月18日至20日在冷春港实验室举行，以从头开始，整个基因组植物测序，预期在不久的将来可以发展的下一代测序的现状，然后确定这些令人兴奋的技术需要哪些进步来允许这些令人兴奋的技术用于进行整个复合植物基因组的Novo Novo测序。 这次会议将汇集利益相关者在高通量测序和基因组学，植物生物学，生物信息学和数据库中的广泛专业知识。会议成果将以报表的形式由参与者制定，这些报告将提交给基因组研究。