EAGER Collaborative Research: Optimizing RNA binding and detection for use in the Capillary Waveguide Biosensor ESP module for automated, in situ microbial process studies

EAGER 协作研究：优化 RNA 结合和检测，用于毛细管波导生物传感器 ESP 模块，用于自动化原位微生物过程研究

• 批准号: 1133999
• 负责人: Paul Kemp
• 金额: $ 2.3万
• 依托单位: University of Hawaii
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-15 至 2013-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1133999&HistoricalAwards=false
• 关键词: EAGER; Collaborative; Research; Optimizing; RNA

In spite of the urgent need for better tools, automated, in situ methods for the determination of the presence, abundance and activity of marine microbes are still in the development stages. The Capillary Waveguide Biosensor (CWB) will potentially make possible long-term, in situ, unattended detection of microorganisms and monitoring of microbial processes. This is a renewal of the PI's EAGER proposal. They were able to complete two of the three tasks originally proposed. With this addition funding they will complete the third task, which was to optimize hybridization protocols and achieve a dynamic range satisfactory for use in real-world studies.Broader Impacts: The potential for training students at graduate, undergraduate and high school levels in this project is particularly interesting and exciting, because it will offer students the opportunities to cross-train in microbial ecology and engineering. Also, education and training of minorities and exposure of the general public to science are two other likely benefits of this project. Participation of C-MORE students is great. If molecular techniques are going to be used more frequently in the future, students are going to have to get used to the technology.

尽管迫切需要更好的工具，但自动化的原位方法来确定存在，但海洋微生物的丰度和活动仍处于开发阶段。毛细血管波导生物传感器（CWB）有可能使长期的原位检测微生物和对微生物过程的监测。这是PI渴望的提议的续签。他们能够完成最初提出的三个任务中的两项。通过这项补充资金，他们将完成第三项任务，即在现实世界研究中优化杂交协议并实现令人满意的动态范围。BRODER的影响：该项目中培训研究生，本科和高中级学生的学生的潜力特别有趣且令人兴奋，因为它将为学生提供微生物生态学和工程学的机会。此外，对少数民族的教育和培训以及公众对科学的接触是该项目的另外两个可能的好处。 C-More学生的参与很棒。如果将来将更频繁地使用分子技术，那么学生将不得不习惯该技术。