IDBR: Development of a Spectroscopic Instrument for the Study of Vibrational Relaxation and Local Motion in Biomolecular Systems

IDBR：开发用于研究生物分子系统中振动弛豫和局部运动的光谱仪器

• 批准号: 1062419
• 负责人: Gary Blanchard
• 金额: $ 27.51万
• 依托单位: Michigan State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1062419&HistoricalAwards=false
• 关键词: IDBR; Development; Spectroscopic; Instrument; Study

AbstractThis proposal is for the construction of an instrument that will provide previously unobtainable information on the organization and dynamics of plasma membranes, biomimetic structures, and light harvesting proteins. The dissipation of energy within lipid bilayers and light harvesting proteins is not well understood but is thought to mediate the function of these two different classes of biomolecular systems. One of the broad biological issues this project addresses is the fundamental, molecular basis for the formation of lipid raft structures, and why such structures vary with the concentrations and identities of the bilayer constituents. Understanding the molecular structural basis for lipid raft organization requires that the viscoelastic properties and intermolecular interactions of the lipid bilayer constituents can be measured. The proposed instrumentation will allow for the measurement of molecular motion and thermal energy flow in biomolecular systems. This project entails designing, constructing, and characterizing an instrument that will apply stimulated-emission spectroscopy to the study of structure and dynamics of biological membranes and proteins. The instrument will reveal how the lipid and protein components of the mammalian cell membrane interact to yield functional assemblies that are responsible for energy transduction, transmembrane transport, and molecular recognition. The instrument will characterize the lipid-lipid and lipid-protein interactions that control the fluidity of the lipid bilayer assembly and the flow of thermal energy between components that serves as the driving force for chemical reactions and molecular motion.The instrument will employ tunable picosecond lasers in a two-color pump?probe detection scheme to examine vibrational energy-transfer and fast molecular-scale motions in bilayer membranes and light harvesting proteins. Such measurements have not been possible before. The detection system is phase-sensitive and shot-noise-limited to measure transmission changes of one part in 107. Because the reaction dynamics underlying the formation and decay of short-lived complexes in membranes are controlled by vibrationally activated barrier-crossing processes, the information obtained with the proposed instrument is crucial to reaching an understanding of the dynamics associated with spatially heterogeneous structures, including lipid rafts and proteins. The creation of broadly accessible instrumentation that advances the state of the art in the measurement of lipid bilayer properties and dynamics will have a major impact on the MSU and regional scientific communities as well as on the global scientific community. The PI and co-PIs collaborate with faculty in a host of other MSU departments (e.g. Biochemistry and Molecular Biology, Food Safety and Toxicology, Cell and Molecular Biology, Microbiology and Molecular Genetics), faculty from nearby institutions (e.g. Saginaw Valley State University, Western Michigan University) and from international institutions (e.g. University of Warsaw (Poland), University of Bath (UK), National University of Singapore, and Shaanxi Normal University (PRC)). They also collaborate with Federal research organizations such as the US Army Engineer Research and Development Center in Champaign, IL. Broadening inclusion of under-represented groups in science is critically important. Michigan State University has multiple programs in place to connect with under-represented groups at the high school (MSU High School Honors Science/Math/Engineering Program (HSHSP), ACS Project SEED), undergraduate (National Organization of Black Chemists and Chemical Engineers (NOBCChE), DREW/TAC Program), graduate (NOBCChE, MSU African-American, Latino(a)/Chicano(a), Asian/Pacific American, and Native American (ALANA) Program) and post-graduate levels (MSU sponsored minority post-doctoral fellowships). The PI and the co-PIs have collaborated with these programs on several levels and continue to strive to provide students from all groups with hands-on research opportunities and mentoring.

Abstrack This Thiss提案是用于构建一种仪器，该仪器将提供有关质膜，仿生结构和光收集蛋白质的组织和动态的先前无法获得的信息。 脂质双层中能量的耗散和光收集蛋白尚不清楚，但被认为可以介导这两种不同类别的生物分子系统的功能。 该项目解决的广泛生物学问题之一是形成脂质筏结构的基本，分子基础，以及为什么这种结构随双层成分的浓度和身份而变化。 了解脂质筏组织的分子结构基础要求可以测量脂质双层成分的粘弹性和分子间相互作用。 提出的仪器将允许测量生物分子系统中的分子运动和热能流。该项目需要设计，构建和表征一种将应用刺激的发射光谱仪的仪器来研究生物膜和蛋白质的结构和动力学。 该仪器将揭示哺乳动物细胞膜的脂质和蛋白质成分如何相互作用，以产生负责能量转导，跨膜转运和分子识别的功能组件。 该仪器将表征控制脂质双层组装的流动性的脂质脂质和脂质 - 蛋白质相互作用，以及在组件之间作为化学反应和分子运动的驱动力之间的热能流动。该仪器将在两位探测器中使用可调的picosecond激光器来检查双 - 探测器的探测器探测器的磁孔和快速摩擦，并快速地进行vibrational contection crom-crom-crom-crom-crom-crom-crom-cotiration Insor-crom-coltibor-coltibor-coltion crom-cotion-fibrational-coltibor-coltion crom-ofor-coltion crom-膜和光收集蛋白。 以前没有可能进行此类测量。 检测系统是相敏感和射击量限制的，可在107中测量一个部分的传播变化。因为膜中短暂寿命复合物的形成和衰减的反应动态受振动激活的屏障跨过程的控制，因此与拟议的仪器获得的信息至关重要蛋白质。在脂质双层属性和动态的测量中，创建可及时可访问的仪器将对MSU和地区科学社区以及全球科学界产生重大影响。 PI和Co-Pis与其他许多密西根州立大学（例如生物化学和分子生物学，食品安全和毒理学，细胞和分子生物学，微生物学和分子遗传学），来自附近机构的教师，附近机构（例如，萨吉诺瓦利州立大学，密歇根州的大学）（来自国际浴场）（E.G。 （英国），新加坡国立大学和Shaanxi师范大学（PRC））。 他们还与伊利诺伊州香槟的美国陆军工程师研发中心等联邦研究组织合作。扩大科学中代表性不足的群体的包容至关重要。 Michigan State University has multiple programs in place to connect with under-represented groups at the high school (MSU High School Honors Science/Math/Engineering Program (HSHSP), ACS Project SEED), undergraduate (National Organization of Black Chemists and Chemical Engineers (NOBCChE), DREW/TAC Program), graduate (NOBCChE, MSU African-American, Latino(a)/Chicano(a), Asian/Pacific American,和美洲原住民（Alana）计划）和研究生级别（MSU赞助了少数族裔博士后奖学金）。 PI和Co-Pis已经在多个层面上与这些计划合作，并继续努力为来自所有团体的学生提供动手研究机会和指导。