Heme uptake by marine bacteria: A molecular-level study of an oceanic iron recycling pathway

海洋细菌吸收血红素：海洋铁循环途径的分子水平研究

基本信息

批准号：
1061068
负责人：
Katherine Barbeau
金额：
$ 35.27万
依托单位：
University of California-San Diego Scripps Inst of Oceanography
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2011
资助国家：
美国
起止时间：
2011-04-01 至 2016-03-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1061068&HistoricalAwards=false
关键词：
Heme uptake marine bacteria molecular

项目摘要

Understanding the mechanisms of assimilation of nutrients by marine microorganisms is important to marine science, especially for the micronutrient iron (Fe), which is often present in limiting concentrations in marine surface waters. Much of the Fe is ocean water is complexed to organic ligands and recent studies have examined the capabilities of marine bacteria to assimilate these different forms of Fe. One potential source of Fe is through the assimilation of heme, a Fe-containing biochemical, and it has been recently shown that marine bacteria have transport systems for heme uptake. This project is therefore aimed at examining if Fe is assimilated as heme and whether this is an important pathway for iron accumulation by marine microorganisms. The potential for heme uptake will be examined in laboratory culture studies. Genetic tools and manipulations will be used to identify the uptake mechanisms, demonstrate their activity and show whether this is an important assimilation pathway. These approaches will also be used to examine the extent of this pathway in natural marine microbial communities.This proposal will integrate its activities with the NSF-funded Center for Ocean Science Education Excellence - California (COSEE CA) and with Scripps Educational Alliances to extend the research impact to K-12 public education. A Research Experiences for Teachers relationship has already been established through these interactions with teachers at University City High School in San Diego. This research will also support for a graduate student whose PhD thesis will focus on this research. The scientific broader impacts of this research relate to understanding the ocean biogeochemistry of Fe and the chemical-biological linkages, and the role of limiting nutrients on the global biogeochemistry of the ocean.

了解海洋微生物同化营养物质的机制对于海洋科学非常重要，特别是对于微量营养素铁 (Fe)，它在海洋表层水中的浓度通常有限。海水中的大部分铁与有机配体络合，最近的研究检查了海洋细菌吸收这些不同形式铁的能力。铁的一个潜在来源是通过血红素（一种含铁的生化物质）的同化，最近表明海洋细菌具有吸收血红素的运输系统。因此，该项目旨在研究铁是否被同化为血红素，以及这是否是海洋微生物积累铁的重要途径。血红素吸收的潜力将在实验室培养研究中进行检查。遗传工具和操作将用于识别摄取机制，证明其活性并表明这是否是重要的同化途径。这些方法还将用于检查该途径在天然海洋微生物群落中的范围。该提案将其活动与 NSF 资助的加利福尼亚州海洋科学教育卓越中心 (COSEE CA) 和斯克里普斯教育联盟相结合，以扩展研究对 K-12 公共教育的影响。通过与圣地亚哥大学城高中教师的互动，已经建立了教师研究经验关系。这项研究还将支持一名研究生，其博士论文将重点关注这项研究。这项研究的更广泛的科学影响涉及了解铁的海洋生物地球化学和化学生物联系，以及限制营养物对海洋全球生物地球化学的作用。