Collaborative Research: Multiple Analytical Window Electrochemical Techniques and Meta-Omics Applied to Studies of Iron Recycling and Iron-Binding Ligands in the Ocean

合作研究：多分析窗口电化学技术和元组学应用于海洋中铁回收和铁结合配体的研究

• 批准号: 1558841
• 负责人: Katherine Barbeau
• 金额: $ 36.98万
• 依托单位: University of California-San Diego Scripps Inst of Oceanography
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-04-01 至 2021-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1558841&HistoricalAwards=false
• 关键词: Collaborative; Research; Multiple; Analytical; Window

The importance of iron (Fe) as a limiting micronutrient in the ocean is a relatively new discovery. And while critical advances have been made in understanding the marine iron cycle, a full description remains elusive due to its biogeochemical complexity and the low concentrations of iron in the ocean. The research funded in this project seeks to advance our understanding of the chemical forms of Fe in seawater. This will be done by first refining a novel, selective electrochemical analytical method for looking at Fe at natural concentrations in seawater and then relating the resulting data to the genetic composition of the microbial communities responsible for creating the organic molecules that control the fate and chemical form of Fe in the ocean. We now know that iron is an incredibly important micronutrient to biological communities, and this new approach of linked genomic and analytical data has the potential to provide important insights that will significantly advance the study of iron composition in the ocean. This project will also support two graduate students at Scripps Institute of Oceanography, both participating in a new outreach program with 6th graders initiated by the Birch Aquarium at Scripps. The importance of iron as a limiting micronutrient in the ocean has become a topic of avid research, and there have been many advances in the field. However, characterizations of iron speciation in seawater are still broad by nature due to the lack of precision in the electrochemical analytical techniques. Competitive ligand exchange - adsorptive cathodic stripping voltammetry (CLE-ACSV) has been a commonly used technique to study iron speciation in seawater. This research seeks to improve this method by applying CLE-ACSV at multiple analytical windows (MAW) to increase resolution at the strong and weak ends of the iron-binding ligand spectrum. Additionally, by relating data from this technique to data on the metagenomic composition of the resident microbial community, the work should be able to make meaningful connections between the microbial communities that produce the organic ligands that we now know dominate iron speciation, solubility, and uptake in the ocean. This research has the potential to make a significant contribution to the current understanding of iron cycling in seawater, and it will help us to better understand the larger global ocean biogeochemical processes that control the distribution and availability of iron and its controls on global productivity.

铁（FE）作为海洋中有限的微量营养素的重要性是一个相对较新的发现。尽管在理解海洋铁循环方面已经取得了关键的进步，但由于其生物地球化学的复杂性和海洋中铁的低浓度，完整的描述仍然难以捉摸。该项目资助的研究旨在促进我们对海水中铁的化学形式的理解。这将是通过首先完善一种新型的选择性电化学分析方法来完成的，用于在海水中的天然浓度上查看Fe，然后将所得数据与负责创建有机分子的微生物群落的遗传组成联系起来，以控制FE中FE的命运和化学形式。现在，我们知道铁是生物群落的一种非常重要的微量营养素，这种新的基因组和分析数据的方法有可能提供重要的见解，从而大大推动对海洋中铁的研究的研究。该项目还将支持Scripps海洋学研究所的两名研究生，他们都参加了一项新的外展计划，由Scripps的Birch Aquarium发起的6年级学生。铁作为海洋中有限的微量营养素的重要性已成为一个狂热的研究的话题，并且该领域已经取得了许多进步。但是，由于电化学分析技术缺乏精确性，海水中铁物种形成的特征仍然很广。 竞争性配体交换 - 吸附性阴极剥离伏安法（CLE -ACSV）一直是一种研究海水中铁物种形成的通用技术。这项研究旨在通过在多个分析窗口（MAW）上应用CLE-ACSV来提高这种方法，以增加铁结合配体谱的强和弱端的分辨率。此外，通过将该技术的数据与居民微生物群落的元基因组组成的数据联系起来，这项工作应该能够在产生我们现在知道的有机配体的微生物群落之间建立有意义的联系，而我们现在知道这些有机配体在海洋中占主导地位的铁物质，溶解度和吸收。这项研究有可能为当前对海水中铁循环的理解做出重大贡献，它将帮助我们更好地了解控制铁及其对全球生产力的控制的较大全球海洋生物地球化学过程。