The GRIK-SnRK1 Protein Kinase Cascade And Its Potential Role In Regulating TCP Transcription Factors In Arabidopsis

GRIK-SnRK1 蛋白激酶级联及其在调节拟南芥 TCP 转录因子中的潜在作用

• 批准号: 1052218
• 负责人: Linda Hanley-Bowdoin
• 金额: $ 70.5万
• 依托单位: North Carolina State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2016-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1052218&HistoricalAwards=false
• 关键词: GRIK; SnRK1; Protein; Kinase; Cascade

Intellectual Merit: The TCPs constitute a large family of plant-specific transcription factors that are defined by a conserved basic helix-loop-helix domain. They fall into two classes with Class I TCPs promoting cell proliferation, and Class II members promoting leaf and floral differentiation. TCPs are sequence-specific DNA binding proteins that bind to shared or overlapping motifs as homo- or heterodimers. Given the complexity of the TCP family and its potential to function combinatorially, it is likely to be governed by an intricate array of regulatory mechanisms. The overall goal of the project is to examine the role of the GRIK-SnRK1 protein kinase cascade in TCP regulation. Although SnRK1 is best known for controlling and coordinating carbon metabolism and energy balance in plants, there is increasing evidence that it also plays an essential role in plant development and controls plant gene expression by phosphorylating transcription factors. GRIK, which activates SnRK1, only accumulates in young plant tissues, cultured cells and geminivirus-infected cells, all of which replicate plant chromosomal DNA. A subset of Arabidopsis TCPs are phosphorylated by the GRIK-SnRK1 kinase cascade, and Class I TCP binding motifs are enriched in SnRK1-down regulated genes. The project will characterize GRIK-SnRK1 target residues of selected TCPs and examine the consequence of altering their phosphorylation status on TCP function. This research represents an important first step in understanding how TCPs are regulated by phosphorylation and the role of post-translational mechanisms in modulating this unique transcription factor family in plants. A better understanding of TCP regulatory mechanisms and the role of the GRIK-SnRK1 cascade in TCP control could lead to the development of new strategies for modulating plant growth, especially under metabolic stress conditions. Broader Impacts: The combined application of molecular, cellular and biochemical techniques to TCP phosphorylation provides a unique training opportunity for undergraduate, graduate and senior researchers. The PIs will work together to foster intellectual and collaborative interactions between their laboratories to ensure that participants are broadly trained and develop the skills necessary to apply integrated approaches to complex biological problems. Project participants will also work with local educators and the NC Museum of Life and Science to offer a two-day workshop based on a genetic "Science in a Suitcase" kit for middle school teachers. The kit is designed as a supplemental teaching aid for teachers embarking on genetics content in the middle school grades and is aligned to the NC Standard Course of Study to ensure maximum potential use. Workshop topics will include kit contents, the NC Standard Course of Study requirements, classroom implementation, overview of genetic science at the middle grades level, and assessment of learning strategies. The outreach project has the potential to reach 3,600 middle school students in NC Granville and Durham Counties, both of which have large numbers of minority and economically disadvantage students. The broader impacts include engaging 30 middle grades math and science teachers in the workshops, providing follow-up support through the Museum staff, and providing the kit at no-cost to participating teachers during the following academic year.

智力优点：TCP构成了由植物特异性转录因子的大家族，这些因子由保守的基本螺旋 - 环螺旋结构域定义。它们分为两类I类TCP，促进细胞增殖，以及促进叶子和花卉分化的II类成员。 TCP是序列特异性DNA结合蛋白，与共享或重叠基序结合为同二聚体或异二聚体。鉴于TCP家族的复杂性及其在合并方面的功能的潜力，因此它可能受到复杂的调节机制的控制。该项目的总体目标是检查Grik-SNRK1蛋白激酶级联反应在TCP调节中的作用。尽管SNRK1以控制和协调植物的碳代谢和能量平衡而闻名，但越来越多的证据表明，它在植物发育中也起着至关重要的作用，并通过磷酸化转录因子来控制植物基因的表达。激活SNRK1的Grik仅在年轻的植物组织，培养的细胞和双子病毒感染的细胞中积聚，所有这些细胞都复制了植物染色体DNA。拟南芥TCP的子集被Grik-SnRK1激酶级联反应磷酸化，I类TCP结合基序富含SNRK1降低的调节基因。该项目将表征所选TCP的Grik-SNRK1目标残基，并检查改变其TCP功能上其磷酸化状态的结果。这项研究是理解TCP如何受磷酸化调节以及翻译后机制在调节植物中这种独特的转录因子家族中的作用的重要第一步。更好地了解TCP调节机制以及Grik-SNRK1级联对于TCP控制中的作用，可能会导致发展调节植物生长的新策略，尤其是在代谢应激条件下。更广泛的影响：分子，细胞和生化技术在TCP磷酸化中的联合应用为本科，研究生和高级研究人员提供了独特的培训机会。 PI将共同努力，以促进其实验室之间的智力和协作互动，以确保对参与者进行广泛培训，并发展将综合方法应用于复杂的生物学问题所需的技能。项目参与者还将与当地教育者和北卡罗来纳州生活与科学博物馆合作，为中学老师提供基于遗传“科学”套件的遗传“科学”的为期两天的研讨会。该套件被设计为一种补充教学援助，适用于在中学成绩中介绍遗传内容的教师，并与NC标准研究课程保持一致，以确保最大的潜在用途。研讨会主题将包括套件内容，NC标准学习要求，课堂实施，中级遗传科学概述以及学习策略的评估。该外展项目有可能吸引北卡罗来纳州格兰维尔和达勒姆县的3,600名中学生，这两个县都有大量的少数族裔和经济上的不利学生。更广泛的影响包括让30年级的数学和科学教师参与讲习班，通过博物馆工作人员提供后续支持，并在接下来的学年以无成本提供套件为参与的教师提供。