Initiation of Geminivirus DNA Replication

双生病毒 DNA 复制的启动

基本信息

批准号：
9506038
负责人：
Linda Hanley-Bowdoin
金额：
$ 32.9万
依托单位：
North Carolina State University
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1995
资助国家：
美国
起止时间：
1995-08-01 至 1998-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9506038&HistoricalAwards=false
关键词：
Initiation Geminivirus DNA Replication

Initiation Geminivirus DNA Replication

项目摘要

9506038 Hanley-Bowdoin Geminiviruses constitute a unique family of eucaryotic DNA viruses with single-stranded genomes that replicate through a rolling circle mechanism in the nuclei of infected plant cells. They have small genomes, encode one protein that is essential for their replicatoin, and recruit the replication machineryof their hosts. These features make geminiviruses excellent model systems for studying eucaryotic DNA replication, an area of study that has not been pursued extensively in plant systems. Study of plant DNA replication and its integration into the cell division cycle will provide valuable insight into the universal mechanisms that underlie these processes in all organisms and may reveal important differences that are responsible for the fundamentally different developmental pathways of plants and animals. This research will study the events that lead to initiation of geminivirus replication, and will provide a rare opportunity to examine the mechanism of rolling circle replication in a eucaryotic system. It will also from the foundation of our long term research goals to identify and understand the functions of host proteins in geminivirus and plant nuclear DNA replication. The well-characterized geminivirus, tomato golden mosaic virus (TGMV), will be used for these experiments. The TGMV replication origin has been mapped to an 89-bp sequence. TGMV encodes two proteins for its replication one (AL1) that is absolutely required and a second (AL3) that impacts viral DNA accumulation. The objectives of this research are to examine the sequence and structural requirements for function of a geminivirus minimal origin of replication, to characterize the functions of an essential geminivirus replication protein, to characterize the steps leading to initiation of geminivirus replication. %%% Geminiviruses constitute a unique family of eucaryotic DNA viruses with single-stranded genomes that replicate through a rolling circle mechanism in the nuclei of infected plant ce lls. They have small genomes, encode one protein that is essential for their replicatoin, and recruit the replication machineryof their hosts. These features make geminiviruses excellent model systems for studying eucaryotic DNA replication, an area of study that has not been pursued extensively in plant systems. The goal of this research is to understand the molecular processes that mediate geminivirus replication and to relate these events to plant nuclear DNA replication. Study of plant DNA replication and its integration into the cell division cycle will provide valuable insight into the universal mechanisms that underlie these processes in all organisms and may reveal important differences that are responsible for the fundamentally different developmental pathways of plants and animals. ***

9506038 Hanley-Bowdoin Geiniviruses构成了独特的桉树DNA病毒家族，具有单链基因组，通过感染植物细胞的核中的滚动圆形机制复制。它们具有小的基因组，编码一种对复制蛋白至关重要的蛋白质，并募集宿主的复制机械。这些功能使双子病毒出色的模型系统用于研究桉树DNA复制，这是一个研究领域，在植物系统中尚未广泛追求。研究植物DNA复制及其在细胞分裂周期中的整合将提供对所有生物中这些过程的普遍机制的宝贵洞察力，并可能揭示出重要的差异，这些差异是造成动植物的根本不同发育途径的原因。这项研究将研究导致双子病毒复制启动的事件，并将提供难得的机会来检查桉树系统中滚动圆复制的机制。它还将从我们的长期研究目标的基础上识别和了解双子座病毒和植物核DNA复制中宿主蛋白的功能。这些实验将使用良好特征的双子病毒，番茄黄色镶嵌病毒（TGMV）。 TGMV复制原点已映射到89 bp序列。 TGMV编码两种蛋白质的复制一个（AL1），这是绝对必需的，第二个（AL3）会影响病毒DNA的积累。这项研究的目的是研究双子病毒最小复制起源的功能的序列和结构要求，以表征必需的双子病毒复制蛋白的功能，以表征导致Gexinivivivivirus Replication的步骤。 %% %% GEMINIVIRUS构成了一个独特的桉树DNA病毒家族，具有单链基因组，通过感染植物CE LLS核中的滚动圆形机制复制。它们具有小的基因组，编码一种对复制蛋白至关重要的蛋白质，并募集宿主的复制机械。这些功能使双子病毒出色的模型系统用于研究桉树DNA复制，这是一个研究领域，在植物系统中尚未广泛追求。这项研究的目的是了解介导GESINIVIVUS复制的分子过程，并将这些事件与种植核DNA复制联系起来。研究植物DNA复制及其在细胞分裂周期中的整合将提供对所有生物中这些过程的普遍机制的宝贵洞察力，并可能揭示出重要的差异，这些差异是造成动植物的根本不同发育途径的原因。 ***