Newly engineered ZnO nanoplatforms and their initial evaluation in ultrasensitive biomedical marker detection

新设计的 ZnO 纳米平台及其在超灵敏生物医学标记物检测中的初步评估

• 批准号: 1042735
• 负责人: Jong-in Hahm
• 金额: $ 22.35万
• 依托单位: Georgetown University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2013-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1042735&HistoricalAwards=false
• 关键词: Newly; engineered; ZnO; nanoplatforms; their

0729541HahmThe objective of the project is to utilize recent advances in nanomaterials to develop improved tools for the detection and quantification of medically relevant protein markers. The research integrates engineering principles of the optical enhancement effect of zinc oxide nanorods (ZnO NRs) in detecting early disease biomarkers. The intellectual merit of the proposed research lies in both fundamental and engineering aspects. The project presents a scheme where a single-step growth will produce ZnO NRs of a uniform size, shape, density, and orientation that are assembled in an array format, directly after synthesis. The project will also ascertain the exact mechanism dictating the important phenomenon of ZnO NR-mediated fluorescence enhancement. The research could then demonstrate that the easily fabricated ZnO NRs will enable high sensitivity protein detection without the need for chemical/enzymatic amplification or specialized instrumentation. These ZnO NR-based protein sensors could have superior detection capabilities than the currently available techniques. The project results could lead to the development of sensitive, low-cost assays of biomedically relevant markers of human disease even at ultratrace levels. The combined growth and assembly process proposed in this research will be applied straightforwardly and conveniently to produce ZnO nanosensor arrays that are compatible with conventional automatic sample handlers and fluorescence readers. This advantage can promote the widespread applications of these nanoplatforms in basic biology, clinical testing, and biomedical research. This versatile platform could not only allow early detection of various disease biomarkers but also facilitate detection of environmental hazards and chemical and biological threats.The proposed research does have a potential to advance discovery and understanding fluorescence detection capability of ZnO nanorods. The project includes the students at graduate and undergraduate levels in both chemical and biomedical engineering.

0729541HAHM该项目的目标是利用纳米材料的最新进展来开发改进的工具，以检测和定量医学上相关的蛋白质标记物。该研究结合了氧化锌纳米棒（ZnO NRS）光学增强作用的工程原理，以检测早期疾病生物标志物。拟议研究的智力优点在于基本和工程方面。该项目提出了一个方案，其中单步生长将产生统一大小，形状，密度和方向的ZnO NR，并在合成后直接以阵列格式组装而成。该项目还将确定决定ZnO NR介导的荧光增强的重要现象的确切机制。然后，这项研究可以证明易于制造的ZnO NRS将无需化学/酶促扩增或专业仪器而实现高灵敏度蛋白检测。这些基于ZnO NR的蛋白质传感器可以比当前可用的技术具有卓越的检测能力。该项目的结果可能会导致敏感的低成本测定法对人类疾病的生物医学相关标记，即使在超级阶段水平上也是如此。这项研究中提出的合并生长和组装过程将直接，方便地应用于与常规自动样品处理程序和荧光读取器兼容的ZnO纳米传感器阵列。这种优势可以促进这些纳米平台在基本生物学，临床测试和生物医学研究中的广泛应用。这个多功能平台不仅可以尽早发现各种疾病生物标志物，而且还可以促进对环境危害以及化学和生物学威胁的检测。拟议的研究确实有可能提高发现和了解ZnO纳米棒的荧光检测能力。该项目包括化学和生物医学工程研究生和本科生的学生。