Collaborative Research: EAGER: Genomic insights into microbial mat diveristy and Proterozoic geobiology

合作研究：EAGER：微生物垫多样性和元古代地球生物学的基因组见解

• 批准号: 1035955
• 负责人: Gregory Dick
• 金额: $ 7.85万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-15 至 2014-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1035955&HistoricalAwards=false
• 关键词: Collaborative; Research; EAGER; Genomic; insights

Cyanobacterial mats were critical in the evolution of Earth?s chemistry and biology. They have long been recognized as drivers of the ?great oxidation event? (GOE), and may have also perpetuated a prolonged period of intermediate redox state in the early Earth's time period call the Proterozoic. However, the types of cyanobacteria that could have mediated such geochemical transitions are poorly understood because whereas Proterozoic oceans were characterized by low oxygen, stratified and/or fluctuating redox conditions, oxygen is required by most modern cyanobacteria studied to date. Further, traditional tools used for study of microbial mats provide limited information regarding metabolic or physiological diversity of cyanobacteria. In this proposal, the PIs propose an exploratory project that will combine cutting-edge single-cell and community genome sequencing technologies with elemental and isotopic geochemistry to address questions focused on facultatively oxygenic/anoxygenic cyanobacteria dominating modern microbial mats that are excellent analogs of Proterozoic mat ecosystems. This EAGER proposal is motivated by two recent, disparate developments that together provide unique opportunities to investigate metabolically versatile cyanobacteria: (i) discovery of an easily accessible modern mat ecosystem in northern Lake Huron that is a novel analog of Proterozoic geochemistry and biology, and (ii) the advent of new genome sequencing technologies that enable unprecedented insights into uncultivated single cells as well as whole microbial communities.Broader Impacts: Undergraduate students will conduct the primary research focused on physiology (GVSU) and geochemistry (UM) and a PhD student will lead the genomics effort (UM). The discovery-driven and exploratory nature of this project provides outstanding opportunities for translating research into educational opportunities and public outreach. The PIs will take advantage of video resources (underwater dive footage, microscopic cell separations), time-lapse photography of motility, and recent coverage on the Discovery Channel (http://watch.discoverychannel.ca/daily-planet/september-2009/daily-planet-september-22-2009/#clip216315) and local news stations to develop multi-media materials (?microbes in motion? and ?laser roundup? movies). These will be integrated into outreach activities at UM and GVSU, and geobiology exhibits at TBNMS?s Visitor Center.

蓝细菌垫对于地球化学和生物学的进化至关重要。他们长期以来一直被认为是“大氧化事件”的驱动因素吗？ （GOE），并且可能还使地球早期的中间氧化还原状态长期存在。但是，可能介导这样的地球化学转变的蓝细菌类型尚不清楚，因为proteroyoc海洋的特征是氧气低，分层和/或波动的氧化还原条件，但迄今为止研究的大多数现代氰基菌都需要氧气。此外，用于研究微生物垫的传统工具提供了有关蓝细菌代谢或生理多样性的有限信息。在该提案中，PIS提出了一个探索性项目，该项目将将尖端的单细胞和社区基因组测序技术与元素和同位素地球化学相结合，以解决针对辅助毒性/氧合性cyanobacteria占主导地位的现代微生物占主导地位的问题，以蛋白质含量为蛋白酶含量的蛋白酶含量Ecosystemss。这项渴望的提议是由最近的两个不同的发展发展的动机，共同提供了独特的机会来调查代谢通用性的蓝细菌：（i）在休伦湖北部北部易于访问的现代MAT生态系统中，这是一种新颖的类似物，是一种新型的类似物，是一种良好的单元，以及（II）良好的良好型良好的良好的良好的良好的良好的良好的良好的良好的态度。整个微生物群落的影响：本科生将进行专注于生理学（GVSU）和地球化学（UM）的主要研究，而博士生将领导基因组学工作（UM）。该项目以发现驱动的和探索性的性质为将研究转化为教育机会和公共外展提供了出色的机会。 The PIs will take advantage of video resources (underwater dive footage, microscopic cell separations), time-lapse photography of motility, and recent coverage on the Discovery Channel (http://watch.discoverychannel.ca/daily-planet/september-2009/daily-planet-september-22-2009/#clip216315) and local news stations to develop multi-media材料（运动中的微生物？和？激光综述？电影）。这些将被整合到UM和GVSU的外展活动中，并在TBNMS的游客中心展出。