IDR/Collaborative Research: Magnetic Beads Linked Immunoassay Meets Micro Coulter Counter: Novel Multiplexed Biosensor System for Food Safety

IDR/合作研究：磁珠联动免疫分析与微库尔特计数器的结合：用于食品安全的新型多重生物传感器系统

• 批准号: 1014779
• 负责人: Hongwei Sun
• 金额: $ 10万
• 依托单位: University of Massachusetts Lowell
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-15 至 2014-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1014779&HistoricalAwards=false
• 关键词: IDR; Collaborative; Research; Magnetic; Beads

The research objective of this Interdisciplinary Research (IDR) collaborative project is to research a novel tool/device for rapid, sensitive, and simultaneous detection of major pathogenic bacteria in foods. The approach taken will be magnetic beads-linked immunoassays combined with micro coulter counting technique to achieve simultaneous detection of multiple pathogenic bacteria in foods with high specificity and sensitivity. A number of novel features across bio-, micro-, and sensing technologies are introduced to the proposed system. Specifically this proposal will develop a simple and practical sensor for detecting the most significant food-borne pathogens in the United States (eg. Salmonella, Campylobacter and enterohemorrhagic Escherichia coli O157:H7) in chicken and ground beef. The project's method, once optimized, can potentially be applied for detecting a wide range of other targets such as viruses, toxins, and disease-related biomarkers. If successful, the benefits of this research will include improved food and water safety, which will lead to efficient monitoring and implementation of homeland security, and enhanced public health. In addition, the proposed project offers a truly multidisciplinary training ground for the undergraduate and graduate students involved in the research. The multidisciplinary nature of the research lends itself to dissemination of information in technical as well as non-technical journals and through outreach activities. The award includes year-round undergraduate research projects, utilizing the NSF REU supplement program, UConn's School of Engineering's Da Vinci project (a project geared toward the integration of basic engineering concepts into the post-elementary school classroom), UConn's E2K program to enhance K-12 education, UMass-Lowell's Women in Sciences and Engineering program to uplift the involvement of female minority students, and activities in Homeschoolers Day.

这项跨学科研究（IDR）协作项目的研究目标是研究一种新颖的工具/设备，以快速，敏感和同时检测食物中主要的致病细菌。采用的方法将是通过磁珠连接的免疫测定与微库尔特计数技术相结合的，以同时检测具有高特异性和灵敏度的食物中多种致病细菌。在提出的系统中引入了跨生物，微型和传感技术的许多新型特征。具体而言，该提案将开发一个简单而实用的传感器，用于检测美国最重要的粮食传播病原体（例如沙门氏菌，弯曲杆菌和鸡肉和牛肉牛肉中的肠胃毛虫O157：H7）。该项目的方法一旦进行了优化，就可以可能用于检测许多其他靶标，例如病毒，毒素和与疾病相关的生物标志物。如果成功的话，这项研究的好处将包括改善食品和水安全，这将导致对国土安全的有效监控和实施，并增强公共卫生。此外，拟议的项目为参与研究的本科生和研究生提供了真正的多学科培训场。该研究的多学科性质使自己在技术和非技术期刊中以及通过外展活动传播信息。 The award includes year-round undergraduate research projects, utilizing the NSF REU supplement program, UConn's School of Engineering's Da Vinci project (a project geared toward the integration of basic engineering concepts into the post-elementary school classroom), UConn's E2K program to enhance K-12 education, UMass-Lowell's Women in Sciences and Engineering program to uplift the involvement of female minority students, and activities in家庭学校节。