CAREER: Protein Switches for Molecular Biotechnology

职业：分子生物技术的蛋白质开关

• 批准号: 0965545
• 负责人: David Wood
• 金额: --
• 依托单位: Ohio State University Research Foundation -DO NOT USE
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0965545&HistoricalAwards=false
• 关键词: CAREER; Protein; Switches; Molecular; Biotechnology

David W. WoodBES-0348220The objective of this proposed study is to develop a new family of ligand-triggered self-splicing and self-cleaving proteins with a wide variety of potential applications. In practice, the switch proteins will be fused to various target proteins at the DNA level to generate functionally inactive precursors. The binding of a small molecule ligand by the engineered switch protein will trigger the switch to splice or cleave as desired for a particular application, typically resulting in the activation of the fused target protein. The development of these protein switches will yield insights into the design and behavior of artificial multifunctional proteins, particularly in the area of allosteric domain fusions, and will include aspects of rational as well as evolution-based approaches. Preliminary results imply immediate uses for these protein switches in biosensing and drug discovery, and new technologies in bioseparations are forthcoming. Long term applications include drug delivery, metabolic engineering and even biomolecular computation. The proposed pedagogical materials seek to provide students with information and skills to allow them to make greater contributions at the interface between engineering and biology, and include a high school intern program, the use of learning style evaluations in an introductory engineering course and a seminar series in applied biotechnology.

David W. WoodBES-0348220 这项研究的目的是开发一个新的配体触发自剪接和自切割蛋白家族，具有广泛的潜在应用。 实际上，开关蛋白将在 DNA 水平上与各种靶蛋白融合，生成功能上无活性的前体。 工程化转换蛋白与小分子配体的结合将触发转换以根据特定应用的需要进行剪接或切割，通常导致融合靶蛋白的激活。 这些蛋白质开关的开发将深入了解人工多功能蛋白质的设计和行为，特别是在变构域融合领域，并将包括理性方法和基于进化的方法的各个方面。 初步结果意味着这些蛋白质开关可立即用于生物传感和药物发现，并且生物分离新技术即将推出。 长期应用包括药物输送、代谢工程甚至生物分子计算。 拟议的教学材料旨在为学生提供信息和技能，使他们能够在工程和生物学之间做出更大的贡献，并包括高中实习计划、在入门工程课程和研讨会系列中使用学习风格评估在应用生物技术领域。