CAREER: Protein Switches for Molecular Biotechnology

职业：分子生物技术的蛋白质开关

• 批准号: 0965545
• 负责人: David Wood
• 金额: --
• 依托单位: Ohio State University Research Foundation -DO NOT USE
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0965545&HistoricalAwards=false
• 关键词: CAREER; Protein; Switches; Molecular; Biotechnology

David W. WoodBES-0348220The objective of this proposed study is to develop a new family of ligand-triggered self-splicing and self-cleaving proteins with a wide variety of potential applications. In practice, the switch proteins will be fused to various target proteins at the DNA level to generate functionally inactive precursors. The binding of a small molecule ligand by the engineered switch protein will trigger the switch to splice or cleave as desired for a particular application, typically resulting in the activation of the fused target protein. The development of these protein switches will yield insights into the design and behavior of artificial multifunctional proteins, particularly in the area of allosteric domain fusions, and will include aspects of rational as well as evolution-based approaches. Preliminary results imply immediate uses for these protein switches in biosensing and drug discovery, and new technologies in bioseparations are forthcoming. Long term applications include drug delivery, metabolic engineering and even biomolecular computation. The proposed pedagogical materials seek to provide students with information and skills to allow them to make greater contributions at the interface between engineering and biology, and include a high school intern program, the use of learning style evaluations in an introductory engineering course and a seminar series in applied biotechnology.

David W. Woodbes-0348220这项拟议的研究的目的是开发一个新的，由配体触发的自剪接和自变态的蛋白质，具有各种潜在应用。 实际上，开关蛋白将在DNA水平上融合到各种靶蛋白上，以产生功能不足的前体。 工程开关蛋白通过工程开关蛋白的结合将触发开关以根据需要触发剪接或裂解特定应用，通常导致熔融靶蛋白的激活。 这些蛋白质开关的开发将为人工多功能蛋白的设计和行为提供见解，尤其是在变构域融合的领域，并包括理性和基于进化的方法的各个方面。 初步结果意味着这些蛋白质开关在生物传感和药物发现中的立即用途，而生物序列中的新技术也即将到来。 长期应用包括药物输送，代谢工程甚至生物分子计算。 拟议的教学材料旨在为学生提供信息和技能，以使他们能够在工程和生物学之间的界面上做出更大的贡献，并包括高中实习计划，在入门工程课程中使用学习风格评估以及应用生物技术的研讨会系列。