MRI-R2: Acquisition of Biophysical Instruments for Interdisciplinary Faculty and Student Research

MRI-R2：为跨学科教师和学生研究购买生物物理仪器

基本信息

批准号：
0959681
负责人：
Kumar Sinniah
金额：
$ 20.86万
依托单位：
Calvin University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2010
资助国家：
美国
起止时间：
2010-01-01 至 2012-12-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0959681&HistoricalAwards=false
关键词：
MRI R2 Acquisition Biophysical Instruments

项目摘要

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).With this award from the Major Research and Instrumentation (MRI) program, Professor Kumar Sinniah and his colleagues David E Benson and Amy Wilstermann from Calvin College, and Brad Wallar from Grand Valley State University will acquire an atomic force microscope (AFM), isothermal titration calorimeter (ITC), and differential scanning calorimeter (DSC). The instruments will be used in an array of chemical and biological investigations including: the binding interaction between G-quadruplex DNA with insulin and with topoisomerase I, the thermodynamic parameters that control protein attachment and stability on nanoparticle surfaces, and the protein interactions involved in the regulation of diaphanous related formins. Atomic force microscopy, isothermal titration calorimetry, and differential scanning calorimetry are used to study the binding of protein-protein and protein-ligand complexes from single molecule to bulk ensemble methods. The single molecule interactions will be studied using the atomic force microscope (AFM), while the bulk ensemble characterizations will be carried out using the complementary techniques of isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC). The ITC and DSC provide critical information regarding the stability and binding of protein-protein and protein-ligand interactions and will aid in the interpretation of single molecule kinetic and thermodynamic properties estimated from the AFM studies. Together these instruments will expand the scope of research and training possible, providing essential bioanalytical capabilities to faculty and students at Calvin College and Grand Valley State University (GVSU).

该奖项是根据2009年的《美国复苏与再投资法》（公法111-5）资助的。与主要研究和仪器计划（MRI）计划（MRI）计划，Kumar Sinniah教授，Kumar Sinniah教授和他的同事David E Benson和Calvin College的David E Benson和Amy Wilstermann，以及来自Grand Valley State University的Calvin College，来自Grand Valley State University的Brad Wallar，将获得Atom Ismocic Microscope（AFM Microscope）（AFM），AFM Microscope，AFM Microscoph，AFM Microscome，AFM Microscome，），AFM Microscome，）（ITC）和差分扫描量热仪（DSC）。这些仪器将用于一系列化学和生物学研究，包括：G-四链体DNA与胰岛素与拓扑异构酶I之间的结合相互作用，与纳米粒子表面上蛋白质附着和稳定性的热力学参数，以及涉及射膜相关形式的蛋白质相互作用的热力学参数。原子力显微镜，等温滴定量热法和差扫描量热法用于研究蛋白质 - 蛋白质蛋白和蛋白质 - 配体复合物的结合，从单分子到块状集合方法。将使用原子力显微镜（AFM）研究单个分子相互作用，而将使用等温滴定量热法（ITC）和差分扫描量热量法（DSC）进行批量集合表征。 ITC和DSC提供了有关蛋白质 - 蛋白质和蛋白质配体相互作用的稳定性和结合的关键信息，并将有助于解释从AFM研究中估计的单分子动力学和热力学特性。这些工具将共同扩大研究和培训的范围，为加尔文学院和格兰德谷州立大学（GVSU）的教师和学生提供重要的生物分析能力。