MRI-R2: Acquisition of Biophysical Instruments for Interdisciplinary Faculty and Student Research

MRI-R2：为跨学科教师和学生研究购买生物物理仪器

• 批准号: 0959681
• 负责人: Kumar Sinniah
• 金额: $ 20.86万
• 依托单位: Calvin University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-01-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0959681&HistoricalAwards=false
• 关键词: MRI; R2; Acquisition; Biophysical; Instruments

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).With this award from the Major Research and Instrumentation (MRI) program, Professor Kumar Sinniah and his colleagues David E Benson and Amy Wilstermann from Calvin College, and Brad Wallar from Grand Valley State University will acquire an atomic force microscope (AFM), isothermal titration calorimeter (ITC), and differential scanning calorimeter (DSC). The instruments will be used in an array of chemical and biological investigations including: the binding interaction between G-quadruplex DNA with insulin and with topoisomerase I, the thermodynamic parameters that control protein attachment and stability on nanoparticle surfaces, and the protein interactions involved in the regulation of diaphanous related formins. Atomic force microscopy, isothermal titration calorimetry, and differential scanning calorimetry are used to study the binding of protein-protein and protein-ligand complexes from single molecule to bulk ensemble methods. The single molecule interactions will be studied using the atomic force microscope (AFM), while the bulk ensemble characterizations will be carried out using the complementary techniques of isothermal titration calorimetry (ITC) and differential scanning calorimetry (DSC). The ITC and DSC provide critical information regarding the stability and binding of protein-protein and protein-ligand interactions and will aid in the interpretation of single molecule kinetic and thermodynamic properties estimated from the AFM studies. Together these instruments will expand the scope of research and training possible, providing essential bioanalytical capabilities to faculty and students at Calvin College and Grand Valley State University (GVSU).

该奖项由 2009 年美国复苏和再投资法案（公法 111-5）资助。来自卡尔文学院的 Kumar Sinniah 教授及其同事 David E Benson 和 Amy Wilstermann 获得了重大研究和仪器 (MRI) 项目的奖项和来自大谷州立大学的 Brad Wallar 将购买原子力显微镜 (AFM)、等温滴定量热计 (ITC) 和差示扫描量热计 (DSC)。 这些仪器将用于一系列化学和生物学研究，包括：G-四链体 DNA 与胰岛素和拓扑异构酶 I 之间的结合相互作用、控制纳米颗粒表面上蛋白质附着和稳定性的热力学参数，以及参与纳米颗粒表面的蛋白质相互作用。透明相关福明的调节。 原子力显微镜、等温滴定量热法和差示扫描量热法用于研究蛋白质-蛋白质和蛋白质-配体复合物的结合，从单分子到整体集成方法。单分子相互作用将使用原子力显微镜（AFM）进行研究，而整体系综表征将使用等温滴定量热法（ITC）和差示扫描量热法（DSC）的互补技术进行。 ITC 和 DSC 提供有关蛋白质-蛋白质和蛋白质-配体相互作用的稳定性和结合的关键信息，并将有助于解释 AFM 研究估计的单分子动力学和热力学特性。 这些仪器将共同扩大研究和培训的范围，为加尔文学院和大谷州立大学 (GVSU) 的教师和学生提供必要的生物分析能力。