MRI-R2: Acquisition of a High-Speed Sorting Flow Cytometer for Multi-User Environmental Microbiology Research

MRI-R2：购买用于多用户环境微生物学研究的高速分选流式细胞仪

• 批准号: 0959630
• 负责人: Zackary Johnson
• 金额: $ 47.34万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0959630&HistoricalAwards=false
• 关键词: MRI; R2; Acquisition; Speed; Sorting

This award is funded under the American Recovery and Reinvestment Act of2009 (Public Law 111-5).This Major Research Instrumentation-Recovery and Reinvestment (MRI-R2) award funds the acquisition of a high-speed sorting flow cytometer for multi-user environmental microbiology research at the Duke University Marine Laboratory. Flow cytometry offers distinct advantages over other techniques, including: the ability to count and characterize large numbers of cells in short times (~50,000 cells/sec); the ability to simultaneously measure multiple properties (size, color or specific genetic (DNA/RNA) or protein constituents using probes) of individual cells and therefore populations; and the ability to sort and collect individual cells or populations of cells for additional downstream analyses. Research enabled by this instrument includes the enumeration and genetic and biochemical characterization of marine microbes, the evaluation of marine and freshwater microbes in the context of environmental pollutants, the analysis of cells harvested from higher organisms of interest to conservation, and the study of algae for the renewable and carbon-neutral production of biofuels and animal protein. The cytometer enables modern environmental microbiology research at a satellite campus of Duke University, and provides access and infrastructure for other regional universities with similar research interests. Beyond developing research infrastructure, the instrument facility is integral to teaching at Duke University and neighboring institutions, allowing incorporation of cutting edge techniques in undergraduate, professional masters and graduate student education. A list of undergraduate and graduate courses at Duke University that directly benefit from this facility includes Biological Oceanography, Genetics and Molecular Biology, Environmental Molecular Tools, and Analysis of Ocean Ecosystems among others. The facility is also to be made available for undergraduate independent study research as well as thesis research by graduate students. Results from the studies enabled by the new equipment will be disseminated by student and faculty presentations at regional and national meetings, and through publication in peer-reviewed journals.

该奖项是根据2009年的《美国回收与再投资法》（公法111-5）资助的。这项主要的研究仪器恢复和再投资（MRI-R2）奖授予了对杜克大学海上大型海军陆战队海洋实验室的多用户环境微生物研究的高速分类流式细胞仪的收购。流式细胞仪比其他技术具有不同的优势，包括：在短时间内计数和表征大量细胞的能力（约50,000个细胞/秒）；同时测量单个细胞以及种群的多种特性（大小，颜色或特定遗传（DNA/RNA）或蛋白质成分）的能力；以及分类和收集单个细胞或细胞种群以进行其他下游分析的能力。 该仪器启用的研究包括海洋微生物的枚举，遗传和生物化学表征，在环境污染物的背景下对海洋和淡水微生物的评估，从感兴趣的较高生物到保护的较高生物的细胞分析以及对藻类的研究，对生产生物蛋白质和动物蛋白质蛋白质的可再生和碳含量生产。 该细胞仪可以在杜克大学的卫星校园内实现现代环境微生物学研究，并为具有类似研究兴趣的其他区域大学提供访问和基础设施。除了开发研究基础设施外，该仪器设施是杜克大学和邻近机构教学不可或缺的一部分，还可以在本科，专业硕士和研究生教育中纳入最先进的技术。杜克大学直接受益于该设施的本科和研究生课程清单，包括生物海洋学，遗传学和分子生物学，环境分子工具以及海洋生态系统的分析。该设施还应用于研究生的本科独立研究以及论文研究。新设备所启用的研究的结果将由区域和国家会议上的学生和教职员工演讲传播，并通过在同行评审期刊上出版。