CAREER: Biosensors Based on Electrogenerated Chemiluminescence

职业：基于电生化学发光的生物传感器

• 批准号: 0955878
• 负责人: Wujian Miao
• 金额: $ 66万
• 依托单位: University of Southern Mississippi
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2016-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0955878&HistoricalAwards=false
• 关键词: CAREER; Biosensors; Based; Electrogenerated; Chemiluminescence

In this Career award, funded by the Chemical Measurement and Imaging program of the Division of Chemistry and the EPSCoR office, Professor Miao from the University of Southern Mississippi and his students will conduct research on biosensors based on electrogenerated chemiluminescence (ECL). The focus will be on simultaneous multiplexing ECL detection and quantification of biomolecules in a sample matrix with nanomaterials. The proposed detection scheme may allow for simultaneous measurements of 3-5 analytes without the need for target amplification or the use of enzymes. By using different types of ECL labels (hydrophobic, hydrophilic, and quantum dots), ECL detection can be performed in either organic or aqueous solutions, or in a solid-state format. Tools such as cyclic voltammetry, ECL coupled with scanning electrochemical microscopy, electron paramagnetic resonance spectroscopy, and electrochemical and ECL digital simulations will be used to achieve these objectives. Furthermore, investigation and preparation of nano-thickness Au film or Au nanoparticles (NPs) coatings for transparent electrodes for high performance ECL detection will be carried out. Finally, investigations of the photochemical, electrochemical, and ECL behavior as well as an exploration of sensing applications of templated micro- to nano-scale semiconductive metal chalcogenide films and NPs will be conducted.ECL biosensors may find applications in clinical diagnostics, forensic chemistry, environmental investigations, pharmaceutical studies, and even in sensitive detection of high explosives and biological warfare agents - which is a pertinent issue in global security.Professor Miao's research will be integrated with undergraduate and graduate education. In this context, an analytical ECL lab that is inexpensive, easy to operate, and adaptable to various levels of teaching and learning environments will be developed with an electrochemical instrument manufacture. In addition, in collaboration with colleagues from USM, Professor Miao will integrate instrumental analysis and inorganic as well as physical chemistry experiments into one senior undergraduate chemistry lab. The implementation of an integrative lab will serve as a model for other chemistry departments in the region and provide the opportunity to forge more robust relationships between USM and regional community colleges.

在这个职业奖中，由化学分部和EPSCOR办公室的化学测量和成像计划资助，来自南密西西比大学的Miao教授及其学生将根据电力化学发光（ECL）对生物传感器进行研究。重点将放在带有纳米材料样品基质中生物分子的ECL检测和定量。提出的检测方案可以同时测量3-5个分析物，而无需目标扩增或使用酶。通过使用不同类型的ECL标签（疏水，亲水和量子点），可以在有机或水溶液中或以固态格式进行ECL检测。 ECL诸如环状伏安法，以及扫描电化学显微镜，电子顺磁共振光谱以及电化学和ECL数字模拟的工具将用于实现这些目标。此外，将进行用于高性能ECL检测的透明电极的纳米厚度AU膜或Au纳米颗粒（NPS）涂层的研究和制备。最后，将对光化学，电化学和ECL行为进行研究，以及对模板微尺度至纳米级半导性金属硫化剂膜和NP的传感应用的探索。ECL生物传感器可能会发现在临床诊断，临床诊断化学中的应用，环境调查，药物研究，甚至在对高爆炸物和生物战代理的敏感检测中 - 这是全球安全中的一个相关问题。专业人士MIAO的研究将与本科和研究生教育相结合。在这种情况下，将通过电化学仪器制造开发一个廉价，易于操作且适应各种教学环境的分析ECL实验室。此外，与USM的同事合作，MIAO教授将将工具分析和无机化学和物理化学实验整合到一个高级本科化学实验室中。综合实验室的实施将成为该地区其他化学部门的模型，并提供了机会，以建立USM与区域社区大学之间更加牢固的关系。