Collaborative Research: The Genomic Effects of Selection for Herbicide Resistance in Green Foxtail

合作研究：青狐尾除草剂抗性选择的基因组效应

• 批准号: 0952177
• 负责人: Katrien Devos
• 金额: $ 59.81万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2015-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0952177&HistoricalAwards=false
• 关键词: Collaborative; Research; Genomic; Effects; Selection

PIs: Katrien M. Devos (University of Georgia) and Elizabeth A. Kellogg (University of Missouri - Saint Louis)CoPI: Jeffrey Bennetzen (University of Georgia)Setaria viridis or green foxtail is a ubiquitous weed that was introduced from Eurasia during the past 500 years. It is often found in and around cultivated fields and the extensive herbicide pressure imposed by agricultural weed control has led to the development of herbicide resistant genotypes. Resistance to four different types of herbicides has been identified in S. viridis. Under continued herbicide application, the genome region that carries the resistance will be under strong selection and this is expected to lead to reduced variation at the herbicide resistance locus and surrounding genome region. The project aims to compare the genetic variation at the whole genome level with the variation present in and around three herbicide resistance genes. To achieve this objective, S. viridis accessions will be collected from North America and Canada, where herbicide resistance has previously been observed. S. viridis accessions from around the world will be obtained from national and international germplasm repositories. The plants will be tested for herbicide resistance. Cutting edge molecular techniques, including high-throughput sequencing, will be used to analyze the genetic variation. The project will provide new insights into the diversity of green foxtail worldwide, the history and origin of green foxtail introductions into North America, the nature of the mutations that give rise to herbicide resistance, how the resistance spreads through populations and the effects of intense directional selection on genome evolution. The project provides a vehicle for training students at the undergraduate, graduate and post-doctoral level in independent research. In addition, the project data will be used as hands-on teaching materials in graduate courses taught by the PIs. The project also aims to provide a link between weed scientists, germplasm curators and geneticists with an interest in green foxtail. To raise awareness of the research interests of the different groups and to facilitate collaborations, the project will organize a workshop to discuss available green foxtail collections, maintenance of those stocks, genomic tools and common goals. All sequence data produced by this project will be made publicly available through GenBank. Germplasm can be obtained from the University of Georgia and the USDA-ARS Germplasm Resources Information Network (GRIN) (www.ars-grin.gov).

PIS：Katrien M. Devos（乔治亚州大学）和伊丽莎白·A·凯洛格（伊丽莎白·A·凯洛格（密苏里大学 - 圣路易斯大学）COPI：杰弗里·贝内岑（乔治亚大学）塞塔里亚·维里迪斯大学或绿色foxtail是在过去的50年中从欧洲裔中引入的，这是从欧洲裔中引入的。 它通常在耕地和周围发现，并且由农业杂草控制施加的广泛的除草剂压力导致了耐除草剂基因型的发展。 在维氏链球菌中已经鉴定出对四种不同类型的除草剂的耐药性。 在持续的除草剂应用下，承载抗性的基因组区域将受到强烈选择，预计这将导致除草剂抗性基因座和周围基因组区域的变异减少。 该项目旨在将整个基因组水平的遗传变异与三种除草剂耐药基因的变异进行比较。 为了实现这一目标，将从以前观察到北美和加拿大的北美和加拿大收集葡萄球菌。来自世界各地的S. viridis加入将从国家和国际种质存储库中获得。 这些植物将接受除草剂耐药性测试。 尖端分子技术（包括高通量测序）将用于分析遗传变异。 该项目将为全球绿色foxtail的多样性提供新的见解，绿色foxtail引入北美的历史和起源，引起除草剂抗性的突变的性质，耐药性如何通过人群传播以及强烈的方向选择对基因组进化的影响。该项目为在独立研究的本科，研究生和博士后一级培训学生提供了一种工具。 此外，项目数据将用作PIS教授的研究生课程中的动手教学。 该项目还旨在提供对绿色foxtail感兴趣的杂草科学家，种质策展人和遗传学家之间的联系。 为了提高对不同群体研究兴趣并促进合作的认识，该项目将组织一个研讨会，讨论可用的绿色foxtail收集，维护这些股票，基因组工具和共同目标。 该项目生产的所有序列数据将通过GenBank公开提供。 可以从佐治亚大学和USDA-ARS种质资源信息网络（GRIN）（www.ars-grin.gov）获得种质。