A MAP kinase cascade downstream of ERECTA and ERECTA-like receptors in regulating plant development

ERECTA 和 ERECTA 样受体下游的 MAP 激酶级联调节植物发育

• 批准号: 0950519
• 负责人: Shuqun Zhang
• 金额: $ 54.67万
• 依托单位: University of Missouri-Columbia
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-02-01 至 2014-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0950519&HistoricalAwards=false
• 关键词: MAP; kinase; cascade; downstream; ERECTA

Intellectual merit: Besides cell differentiation, stage-specific and localized cell division is also critical to the formation of normal tissues, organs, and organisms. The highly coordinated cell differentiation and proliferation events during growth and development illustrate the importance of cell-cell communication, which is carried out by cell surface receptors/sensors and intracellular signaling components. In Arabidopsis, ERECTA (ER), a receptor-like kinase, and its homologs (ERL1 and ERL2) play important roles in these processes. Their mutation alters both the architecture and the reproduction of the plant. Similar to er or er/erl1/erl2 mutant plants, loss-of-function of MPK3 and MPK6, two Arabidopsis mitogen-activated protein kinases (MAPKs), or their upstream MAPKKs (MKK4/MKK5) also resulted in similar phenotypes. Genetic analysis demonstrated that the gain-of-function MAPKK transgene can rescue the loss-of-function er or er/erl1/erl2 mutants, suggesting that MPK3/MPK6 function downstream of ER/ERL1/ERL2 receptors. The goal of this project is to understand the molecular mechanism underlying the function of MPK3/MPK6 in the regulation of localized cell division. In addition, the MAPKK kinase(s) upstream of the MKK4/MKK5-MPK3/MPK6 module will be identified. A combinatory approach will be employed to reach these goals. Defining a specific MAPK cascade downstream of the well-characterized ER/ERL1/ERL2 receptor-like kinases is important to our understanding of cell-cell communication. The identification of new components downstream of MPK3/MPK6 in the ER/ERL1/ERL2 pathway will reveal the molecular mechanism underlying their role in the localized cell division. The research will also reveal the functional diversity and signaling specificity of MPK3 and MPK6. The tools generated in this project will have broad applications and will be shared with other groups. Broader impacts: This project will use an integrative approach to understand the roles of a MAPK cascade and its upstream receptor-like kinases in coordinating plant development, which will serve as an excellent training environment for students and postdoctoral fellows. Training in use of integrative approaches to the study of biological phenomena is critical to the advancement of post-genome biology. In addition to scientific methodology, students/post-docs will also receive training in ethics and career development. Students (both graduate and undergraduate) and post-docs from under-represented groups will be actively recruited by participating in institutional programs that reach out to minorities. Post-docs involved in the project will follow a career development plan supported by the research training in the lab, student mentoring experiences, and professional development training. Understanding the signal transduction pathways and the identification of important regulators of plant growth and development could positively impact food/feed/biofuel production, which is important to sustain the increasing world population.

智力价值：除了细胞分化外，阶段特异性和局部细胞分裂对于正常组织、器官和生物体的形成也至关重要。生长和发育过程中高度协调的细胞分化和增殖事件说明了细胞间通讯的重要性，该通讯是由细胞表面受体/传感器和细胞内信号传导成分进行的。在拟南芥中，ERECTA (ER)（一种受体样激酶）及其同源物（ERL1 和 ERL2）在这些过程中发挥着重要作用。它们的突变改变了植物的结构和繁殖。与 er 或 er/erl1/erl2 突变体植物类似，MPK3 和 MPK6、两种拟南芥有丝分裂原激活蛋白激酶 (MAPK) 或其上游 MAPKK (MKK4/MKK5) 的功能丧失也会导致类似的表型。遗传分析表明，功能获得性 MAPKK 转基因可以挽救功能丧失的 er 或 er/erl1/erl2 突变体，表明 MPK3/MPK6 在 ER/ERL1/ERL2 受体下游发挥作用。该项目的目标是了解 MPK3/MPK6 在调节局部细胞分裂中功能的分子机制。此外，还将鉴定 MKK4/MKK5-MPK3/MPK6 模块上游的 MAPKK 激酶。将采用组合方法来实现这些目标。定义已充分表征的 ER/ERL1/ERL2 受体样激酶下游的特定 MAPK 级联对于我们理解细胞间通讯非常重要。 ER/ERL1/ERL2 通路中 MPK3/MPK6 下游新成分的鉴定将揭示其在局部细胞分裂中作用的分子机制。该研究还将揭示 MPK3 和 MPK6 的功能多样性和信号传导特异性。该项目中生成的工具将具有广泛的应用，并将与其他小组共享。 更广泛的影响：该项目将采用综合方法来了解 MAPK 级联及其上游受体样激酶在协调植物发育中的作用，这将为学生和博士后研究员提供良好的培训环境。使用综合方法研究生物现象的培训对于后基因组生物学的进步至关重要。除了科学方法论之外，学生/博士后还将接受道德和职业发展方面的培训。来自代表性不足群体的学生（研究生和本科生）和博士后将通过参与面向少数群体的机构项目来积极招募。参与该项目的博士后将遵循由实验室研究培训、学生指导经验和专业发展培训支持的职业发展计划。了解信号转导途径并识别植物生长和发育的重要调节因子可能会对粮食/饲料/生物燃料生产产生积极影响，这对于维持不断增长的世界人口非常重要。