Functional Genomics of Maize Centromeres

玉米着丝粒的功能基因组学

• 批准号: 0922703
• 负责人: R Kelly Dawe
• 金额: $ 398.93万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-06-15 至 2016-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0922703&HistoricalAwards=false
• 关键词: Functional; Genomics; Maize; Centromeres

PI: R. Kelly Dawe (University of Georgia - Athens)CoPIs: James A. Birchler (University of Missouri - Columbia), Jiming Jiang (University of Wisconsin - Madison), Gernot Presting (University of Hawaii - Manoa), and Jeffrey Ross-Ibarra (University of California - Davis)Centromeres are regions of the genome that organize and regulate chromosome movement. They are key elements in the development of next-generation artificial chromosomes for introducing new traits into crop plants. Yet the biology of centromeres remains poorly understood. The experiments carried out in this project will complete the sequence and assembly of maize centromeres, address known epigenetic limitations associated with employing artificial chromosomes, develop improved strategies for streamlining centromere study and use, and document the diversity of centromeres in maize and allied species. To expand training opportunities in plant genetics, two programs will be initiated. One will provide hands-on training in plant cytogenetics through an Annual Cytogenetics Workshop to be held at the University of Wisconsin. The second will be an international student exchange program between project laboratories and the National Autonomous University of Mexico. Specifically, thirteen students will be funded to travel between universities and work on collaborative projects relating to centromere evolution and function. A web site (www.plantcentromeres.org) will be used to disseminate results from the project and to coordinate outreach activities. In addition, sequence data will be deposited at MaizeGDB (www.maizegdb.org) and GenBank. Germplasm generated will be deposited at the Iowa North Central Regional Plant Introduction Station (Ames, IA).

PI：R。KellyDawe（佐治亚大学 - 雅典）COPIS：James A. Birchler（密苏里大学 - 哥伦比亚大学），Jiming Jiang（威斯康星大学 - 麦迪逊大学 - 麦迪逊大学），夏威夷大学 - 夏威夷大学 - 马诺阿）和杰弗里·罗斯·罗斯·罗斯（Jeffrey Ross -ibarra） 移动。 它们是下一代人工染色体发展的关键要素，用于将新特征引入作物植物。然而，centromeres的生物学仍然知之甚少。 在该项目中进行的实验将完成玉米中心粒的顺序和组装，解决与使用人造染色体相关的已知表观限制，开发改进的策略来简化Centromere研究和使用，并记录玉米和Allied物种中心粒的多样性。 为了扩大植物遗传学的培训机会，将启动两个程序。 人们将通过在威斯康星大学举行的年度细胞遗传学研讨会上提供植物细胞遗传学的动手培训。第二个将是项目实验室和墨西哥国家自治大学之间的国际学生交流计划。 具体来说，将资助13名学生在大学之间旅行，并从事与Centromere Evolution和功能有关的协作项目。 网站（www.plantcentromeres.org）将用于传播项目的结果并协调外展活动。此外，序列数据将沉积在玉米式（www.maizegdb.org）和GenBank上。 产生的种质将沉积在爱荷华州北部中部地区植物介绍站（IA，IA）。