MRI: Acquisition of a Flow Cytometer Instrument for Undergraduate Research

MRI：为本科生研究购买流式细胞仪

• 批准号: 0921524
• 负责人: Jay Brewster
• 金额: $ 10.86万
• 依托单位: Pepperdine University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0921524&HistoricalAwards=false
• 关键词: MRI; Acquisition; Flow; Cytometer; Instrument

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).Pepperdine University has acquired a Cell Lab Quanta SC Flow Cytometer system. The flow cytometer instrument will be utilized in our summer undergraduate research programs and Honor's research program in original, student generated research projects. This instrument will be utilized in a variety of undergraduate research projects carried out in the context of the biology department's current summer program funded in part through a NSF-REU site grant. Acquisition of a flow cytometer will allow us to introduce new concepts into the laboratories of our core cell biology, biochemistry and molecular biology courses, several of our upper-division biology elective courses, and related courses in sports medicine and psychology. Each faculty member associated with this grant is tasked to develop a new undergraduate laboratory teaching module that explores an application of flow cytometry in cell structure or function. The grant will provide undergraduate students with access to the latest equipment for research in molecular and cellular biology. Funds will foster undergraduate research in cell and molecular biology to facilitate interest in graduate studies in cell and molecular biology and also provide training to students interested in employment in biotechnology industries. Specific research programs that will be aided through acquisition of this instrument are: (1) detection of stress signal molecules in pre- and post-apoptotic cells; (2) examining downstream targets in Notch signaling of a mixed population of 7F2 cells; (3) examining changes in levels of phosphorylated and total CaMKII in the hippocampus following neural injury; (4) examining cellular signaling in mammalian vascular smooth muscle under normal and short term exercise training regimes; and (5) examining ploidy and hybridization in chaparral plants. In addition, we will be able to connect undergraduate research and teaching in biology with research and teaching conducted in psychology and sports medicine, thus broadening the interest base and student interconnections between these disciplines within Seaver College at Pepperdine University. Results of the research efforts accomplished from this grant will be available on each investigator's web pages and in peer reviewed publications.

该奖项是根据2009年的《美国回收与再投资法》（公法111-5）资助的。PepperdineUniversity已收购了一个细胞实验室量子量子SC流式细胞仪系统。 流式细胞仪仪器将用于我们的夏季本科研究计划和荣誉研究计划中的原始学生生成的研究项目。 该工具将用于在生物学部门当前的夏季计划的各种本科研究项目中，部分通过NSF-REU网站赠款资助。 获取流式细胞仪将使我们能够将新概念引入我们的核心细胞生物学，生物化学和分子生物学课程的实验室，我们的几个上级生物学选择性课程以及运动医学和心理学的相关课程。 与该赠款相关的每个教师的任务都是开发一个新的本科实验室教学模块，该模块探讨了流式细胞仪在细胞结构或功能中的应用。 该赠款将为本科生提供分子和细胞生物学研究的最新设备。 资金将促进细胞和分子生物学的本科研究，以促进对细胞和分子生物学研究生研究的兴趣，并为对生物技术行业有兴趣的学生提供培训。 通过获取该仪器将有助于的特定研究计划是：（1）检测前后凋亡细胞中应力信号分子； （2）检查7F2细胞混合群体的凹槽信号传导下游靶标； （3）检查神经损伤后海马中磷酸化和总CAMKII水平的变化； （4）在正常和短期运动训练方面检查哺乳动物血管平滑肌的细胞信号传导； （5）检查植物植物中的倍虫和杂交。 此外，我们将能够将生物学的本科研究和教学与心理学和运动医学领域进行的研究和教学联系起来，从而扩大了Pepperdine University在Seaver College内的这些学科之间的兴趣基础和学生的互连。 该赠款完成的研究工作的结果将在每个研究者的网页以及同行评审的出版物中获得。