DISSERTATION RESEARCH: The Genetic Basis of Recombination Rate Variation in House Mice

论文研究：家鼠重组率变异的遗传基础

• 批准号: 0909779
• 负责人: Bret Payseur
• 金额: $ 1.5万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2011-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0909779&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Genetic; Basis; Recombination

Meiosis is a specialized cell division that gives rise to sperm and egg cells that bear half the amount of DNA as other cells in the body. Recombination occurs at the onset of meiosis and is particularly important to the partitioning of DNA into sperm and egg cells. During recombination, chromosomes with high sequence similarity become physically joined and swap pieces of their genetic material. The frequency of recombination and the placement of recombination events along paired chromosomes are highly variable among individuals. Using the house mouse as a model system, this research will combine molecular genetic methods with new techniques for imaging cells undergoing meiosis and the cellular proteins that mediate recombination to identify the genetic factors that control the amounts and chromosomal locations of recombination during meiosis. This project will provide research opportunities for undergraduate students and will support the continued training of a female graduate student in quantitative and statistical methods, an area of genetics in which women are under-represented. Additionally, this project will promote multidisciplinary collaboration among statisticians, evolutionary biologists, and molecular biologists. Finally, understanding the genetic basis of variation in recombination has important applications for health. Aberrant recombination is linked to infertility and sterility, and identifying the genes that contribute to variation in recombination has potential application to these challenges.

减数分裂是一种专门的细胞分裂，会产生精子和卵细胞，其含量是体内其他细胞的一半。重组发生在减数分裂开始时，对于将DNA分配到精子和卵细胞中尤为重要。在重组过程中，具有高序列相似性的染色体会变得物理上加入并交换其遗传物质的部分。重组的频率和沿配对染色体的重组事件的放置频率在个体之间是很大的变化。该研究将使用室小鼠作为模型系统，将分子遗传学方法与经历减数分裂细胞的新技术结合在一起，以及介导重组的细胞蛋白，以确定控制减数分裂过程中重组的量和染色体位置的遗传因子。 该项目将为本科生提供研究机会，并将支持对定量和统计方法的女性研究生的继续培训，这是女性代表不足的遗传学领域。此外，该项目将促进统计学家，进化生物学家和分子生物学家之间的多学科合作。最后，了解重组变异的遗传基础具有重要的健康应用。异常重组与不育和不育有关，并且确定导致重组变化的基因可能对这些挑战的潜在应用。