Collaborative Research: The Genetics of Postmating, Prezygotic Isolation

合作研究：交配后、合子前隔离的遗传学

• 批准号: 0852175
• 负责人: Daniel Howard
• 金额: $ 23.02万
• 依托单位: University of Colorado at Denver-Downtown Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-03-15 至 2012-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0852175&HistoricalAwards=false
• 关键词: Collaborative; Research; Genetics; Postmating; Prezygotic

Research over the past 20 years has shown that trait differences acting after copulationbut before the fusion of sperm and egg nuclei, often termed postmating, prezygotic traitdifferences, play a primary role in reducing gene exchange between populations withina species and between closely related species. The genetic basis of these traitdifferences is poorly understood and thus, constitutes the primary goal of Marshall andHoward's research plan. Specifically, the proposed research focuses on identifying theseminal fluid and sperm proteins that differ, whether in abundance or structure, betweenclosely related ground cricket species that are isolated by postmating, prezygotic traitdifferences. Once identified, gene silencing technology will be used to evaluate thefunction of each protein and its influence on preventing or reducing fertilization betweenspecies.Barriers to fertilization have been intensively studied in the Allonemobius sociuscomplex of crickets, providing an excellent model for evaluating the genes and proteinsthat underlie successful fertilization. Understanding the genetic basis of fertilizationincompatibilities between populations and species can provide insight into a wide-rangeof topics, including the development of infertility within species (including humans) andthe role of sexual selection and sexual conflict in driving the divergence of fertilizationproteins. The project provides training for undergraduate and graduate students andoutreach to K-12 schools.

过去20年的研究表明，在交配后、精子和卵子核融合之前发生的性状差异，通常称为交配后、合子前性状差异，在减少物种内种群之间以及密切相关物种之间的基因交换方面发挥着主要作用。人们对这些性状差异的遗传基础知之甚少，因此构成了马歇尔和霍华德研究计划的主要目标。具体来说，拟议的研究重点是识别通过交配后、合子前性状差异分离出的密切相关的地面蟋蟀物种之间精液和精子蛋白的丰度或结构差异。一旦确定，基因沉默技术将用于评估每种蛋白质的功能及其对阻止或减少物种间受精的影响。受精障碍已在蟋蟀的 Allonemobius 社会复合体中进行了深入研究，为评估其背后的基因和蛋白质提供了一个极好的模型。受精成功。了解种群和物种之间受精不相容性的遗传基础可以深入了解广泛的主题，包括物种（包括人类）内不育的发展以及性选择和性冲突在驱动受精蛋白分化中的作用。该项目为本科生和研究生提供培训，并向 K-12 学校进行推广。