Development of AlGaN Biosensor sensitive in physiological saline

开发对生理盐水敏感的AlGaN生物传感器

• 批准号: 0756594
• 负责人: Stephen Lee
• 金额: $ 35万
• 依托单位: Ohio State University Research Foundation -DO NOT USE
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0756594&HistoricalAwards=false
• 关键词: Development; AlGaN; Biosensor; sensitive; physiological

CBET-0756594S. Lee, Ohio State UniversityThis proposal targets the problems of the Debye length in using FET for detection of biomolecules. Since the distance of detection is limited to 10 nm or less, antibodies have not been used to target biomolecules using FET sensors since antibody length is beyond the Debye length. The investigators have taken the approach of using just the targeting antibody fragment for HEMT. To achieve this goal, the approaches taken include: (1) to identify antibody fragments (scFvs and VHH) that recognize MIG (analyte receptors) and engineer selected scFv and VHH thereof for site specific conjugation to HEMT sensing surfaces; (2) to characterize specificity and sensitivity of MIG-detecting HEMTs decorated with scFvs and VHHs and the chemical and electronic properties of sensing interfaces; (3) to use a control gate or an optimized structure on scaled nano-device for sensitivity enhancement and (4) to establish theoretical models and perform simulations, coupled with experimental results, for fundamental understanding.

CBET-0756594S。 Lee，俄亥俄州立大学该提案针对的是使用 FET 检测生物分子时的德拜长度问题。由于检测距离仅限于 10 nm 或更小，因此抗体长度超出了德拜长度，因此尚未使用 FET 传感器来靶向生物分子。研究人员采取了仅使用 HEMT 靶向抗体片段的方法。为了实现这一目标，采取的方法包括：(1) 鉴定识别 MIG（分析物受体）的抗体片段（scFv 和 VHH），并设计选定的 scFv 和 VHH，以便与 HEMT 传感表面进行位点特异性缀合； (2) 表征用 scFv 和 VHH 修饰的 MIG 检测 HEMT 的特异性和灵敏度以及传感界面的化学和电子特性； （3）在按比例缩放的纳米器件上使用控制门或优化结构来增强灵敏度；（4）建立理论模型并进行模拟，结合实验结果，以实现基本理解。