CAREER: Use of C. Elegans to Identify Alleles and Genotypes that Modulate Severe Anoxia Survival

职业生涯：利用线虫来识别调节严重缺氧生存的等位基因和基因型

• 批准号: 0747391
• 负责人: Pamela Padilla
• 金额: $ 63.96万
• 依托单位: University of North Texas
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-08-01 至 2014-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0747391&HistoricalAwards=false
• 关键词: CAREER; Use; C.; Elegans; Identify

Environmental stress is a fact of life, in that all organisms are exposed to ever changing conditions. Organisms have clearly adapted the capacity to sense and survive changes within their environment, yet if the environmental stress is either prolonged or too severe, the viability of the organism is compromised. Preliminary data supports the idea that oxygen deprivation survival is dependent, at least in part, on the insulin-like signaling pathway (daf-2/daf-16). The central hypothesis of this grant is that the insulin-like signaling pathway modulates specific genes that confer severe anoxia survival. A genetic approach using the model system C. elegans, will be used to identify specific alleles that lead to oxygen deprivation survival. The following will be identified and analyzed: genetic mutants that are resistant to long-term anoxia, gene expression profiles in specific tissues of organisms exposed to oxygen deprivation and the role specific organs and tissue have in response to and survival of oxygen deprivation. These studies will begin to explain, at the genetic level, why some organisms or tissues are more resistant to oxygen deprivation. The proposed educational activities focus on development of discovery-based lessons for the undergraduate genetics lab course and the existing K-7th grade science program at Elm Fork Education Center (EFEC). The development of these lessons will allow students to be actively involved in the process of scientific discovery, gain a better sense of accomplishment, and promote an increased interest in science education. The involvement in the EFEC, a nationally known, highly innovative, inquiry-based science education program will expose thousands of students to scientific discovery.

环境压力是生活中的一个事实，因为所有生物体都暴露在不断变化的条件下。生物体显然已经适应了感知环境变化并生存的能力，但如果环境压力长期或过于严重，生物体的生存能力就会受到损害。初步数据支持这样的观点：缺氧生存至少部分依赖于胰岛素样信号通路 (daf-2/daf-16)。该资助的中心假设是胰岛素样信号通路调节特定基因，从而赋予严重缺氧生存。使用线虫模型系统的遗传方法将用于识别导致缺氧生存的特定等位基因。将识别和分析以下内容：抵抗长期缺氧的基因突变体、暴露于缺氧的生物体特定组织中的基因表达谱以及特定器官和组织对缺氧的反应和生存的作用。这些研究将开始在基因水平上解释为什么某些生物体或组织对缺氧具有更强的抵抗力。拟议的教育活动重点是为本科遗传学实验课程和 Elm Fork 教育中心 (EFEC) 现有的 K-7 年级科学课程开发基于发现的课程。这些课程的开发将使学生积极参与科学发现的过程，获得更好的成就感，并促进对科学教育的兴趣增加。 EFEC 是一项全国知名的、高度创新的、基于探究的科学教育计划，参与该计划将使成千上万的学生接触到科学发现。