Collaborative Research: Resistance of Peptide Degradaton Products in Seawater

合作研究：海水中肽降解产物的抗性

• 批准号: 0726632
• 负责人: Cindy Lee
• 金额: $ 28.59万
• 依托单位: SUNY at Stony Brook
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0726632&HistoricalAwards=false
• 关键词: Collaborative; Research; Resistance; Peptide; Degradaton

The principal source of organic matter in marine systems is from in situ primary production by photosynthetic microorganisms. The bulk of the produced organic matter is remineralized in the water column and sediments, but a small fraction survives diagenesis to be preserved. Of particular importance to global carbon and nitrogen budgets is organic matter's inherent resistance to decay and its transformation into refractory substances, both of which remove it from the reactive biotic and abiotic marine cycles. Little is known, however, about the chemical composition of this refractory, macromolecular organic matter, or mechanisms contributing to its resistance during diagenesis.Scientists from the State University of New York, Stony Brook and Old Dominion University propose to determine the biological and chemical basis for the observed distribution of protein, peptide, and amino acid pools in the marine environment. Specifically, they plan to compare the rates of competing chemical, physical, and biological reactions of peptides to gain a better understanding of the distribution, cycling, and fate of proteinaceous material in seawater. Through the use of state-of-the-art analytical methods (i.e., matrix-assisted laser desorption/ionization mass spectrometry-MALDI-MS, electrospray mass spectrometry coupled to Fourier transform ion cyclotron mass spectrometry-ESI FTICR, 2D nuclear magnetic resonance approaches), the principle investigators plan to synthesize peptides of biological interest and monitor their rates of production and removal. This would lead to observations of the importance of chemical and biological loss mechanisms in various environments with respect to change in light, pH, nutrients, and temperature. As regards broader impacts, results from this study would provide a better understanding of the bioavailability of organic matter to microbes, the mechanisms whereby organic matter is rendered less available in the water column, and how organic matter is preserved. One graduate student and one undergraduate student from the State University of New York, Stony Brook, and one postdoc and one graduate student from Old Dominion University would be supported and trained as part of this project. Results and methods from this project would be integrated into graduate and undergraduate education and training at both universities.

海洋系统中有机物的主要来源来自光合微生物的原位初级生产。 产生的有机物的大部分在水柱和沉积物中被重新矿化，但是一小部分可以保存成岩作用。 对全球碳和氮预算特别重要的是有机物对腐烂的固有抗性及其转化为难治性物质，这两者都将其从反应性生物和非生物海洋周期中消除。 然而，关于这种折磨性，大分子有机物的化学组成，或有助于其在成岩作用期间耐药性的机制。 具体而言，他们计划比较肽的竞争化学，物理和生物学反应的速率，以更好地了解海水中蛋白质材料的分布，循环和命运。通过使用最先进的分析方法（即，基质辅助激光解吸/电离质谱 - 质谱 - 马尔迪 - 摩尔数 - 耦合到傅立叶转化离子循环基因群质谱仪的质谱法，2D核磁共振效果及其速度的兴趣效果） 移动。这将导致观察到各种环境中化学和生物损失机制在光，pH，养分和温度变化方面的重要性。关于更广泛的影响，这项研究的结果将更好地理解有机物对微生物的生物利用度，在水柱中有机物较少可用的机制以及如何保留有机物。作为该项目的一部分，将为纽约州立大学，斯托尼·布鲁克（Stony Brook），斯托尼·布鲁克（Stony Brook），斯托尼·布鲁克（Stony Brook）的一名研究生和一名本科生和一名研究生和一名研究生。 该项目的结果和方法将纳入两所大学的研究生和本科教育和培训中。