Collaborative Research: A Post-Genomic Approach to Synechococcus-grazer Interactions

合作研究：聚球藻-食草动物相互作用的后基因组方法

• 批准号: 0648572
• 负责人: Suzanne Strom
• 金额: $ 25.28万
• 依托单位: Western Washington University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-15 至 2011-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0648572&HistoricalAwards=false
• 关键词: Collaborative; Research; Post; Genomic; Approach

The most common fate of microbes in aquatic environments is to become a meal for a protest grazer. A major flux of carbon in aquatic environments is thus mediated through microbe-protist interactions. The mechanisms by which microbes attempt to avoid grazers likely include morphological, behavioral, and chemical resistance strategies, yet our knowledge of these is primitive and most progress is currently being made on harmful algal bloom species. One of the insights from the availability of multiple cyanobacterial genomes is that different cyanobacteria have very different cell surface structures as well as potentially different modifications of common structures. In addition, potential chemical resistance strategies have been detected in several available genomes. This project will address protest-cyanobacterial interactions with the collaboration of researchers with expertise in protist biology, cyanobacterial ecology, molecular genetics and genomics to examine the range of interactions between protists and cyanobacteria of the genus Synechococcus. The investigators propose to use the recent availability of complete genomes and molecular genetics tools to determine the key cell structures under selection by diverse protist grazers. They will use "shot-gun" approaches (transposon mutagenesis and selection) to identify these structures and use available mutants to investigate specific known cell surface structures. They will also examine the role of some potential chemical defense enzymes, found through whole genome comparisons, in deterring grazing. The proposed research will provide qualitative and quantitative information on the role of various grazer deterrence strategies and new paradigms for understanding grazer-prey interactions.Broader Impacts of the Proposed ResearchThe broader impacts of this work will include the training of undergraduate and graduate students, including those from under-represented groups. Scientific findings will be disseminated broadly though participation in national meetings and publication in peer-review journals. The findings may indicate a mechanistic basis for understanding microbe-grazer interactions, one of the major processes determining carbon cycling in the oceans. The investigators expect to define one or more model but ecologically relevant grazer-cyanobacteria pairs that could be used by the community for future studies. In addition, novel marine natural products that affect eukaryotic cells, with possible biotechnological applications, may be found through these activities.

在水生环境中，微生物最常见的命运是成为抗议格雷泽的一顿饭。因此，在水生环境中，碳的主要通量是通过微生物 - 促销师相互作用介导的。微生物试图避免放牧者的机制可能包括形态学，行为和耐化学策略，但是我们对这些策略的了解是原始的，目前正在对有害藻类开花物种取得大多数进步。来自多个蓝细菌基因组的可用性的见解之一是，不同的蓝细菌具有非常不同的细胞表面结构以及潜在的公共结构修饰。此外，在几种可用的基因组中已经检测到了潜在的耐化学策略。该项目将解决与研究人员在原则生物学，蓝细菌生态学，分子遗传学和基因组学方面的专业知识方面的合作抗议 - 分子的相互作用，以研究生物学生物和蓝细菌之间的相互作用范围。研究人员建议使用最新的完整基因组和分子遗传学工具来确定各种植物生物植物植物的关键细胞结构。他们将使用“射击”方法（转座子诱变和选择）来识别这些结构，并使用可用的突变体研究特定的已知细胞表面结构。他们还将检查通过整个基因组比较发现的一些潜在的化学防御酶在阻止放牧中的作用。拟议的研究将提供有关各种格拉泽威慑策略的作用和新的范式来理解Grazer-Prey互动的作用的定性和定量信息。拟议的研究对这项工作的更广泛影响的影响将包括对本科生和研究生的培训，包括来自代表不足的小组的培训。通过参加同行评审期刊的全国会议和出版物，科学发现将广泛传播。这些发现可能表明理解微生物相互作用的机械基础，这是确定海洋中碳循环的主要过程之一。调查人员希望定义一个或多个模型，但与生态相关的格拉泽 - 斜杆菌对，可以被社区用于未来的研究。此外，可以通过这些活动可以找到影响真核细胞的新型海洋天然产品，并具有可能的生物技术应用。