Molecular and functional analysis of trehalose metabolism during pea seed development

豌豆种子发育过程中海藻糖代谢的分子和功能分析

• 批准号: 178974956
• 负责人: Dr. Hans Weber
• 金额: --
• 依托单位: Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/178974956?language=en
• 关键词: Molecular; functional; analysis; trehalose; metabolism

Seed metabolism must be coordinated with endogenous developmental and exogenous environmental factors. This is thought to be performed by sugar signalling mediated by SnRK1, abscisic acid and the Trehalose (Tre) pathway. Tre and Tre-6-P are minor sugars in plants but represent important signals to integrate and coordinate metabolism with development. In this project, we are planning a molecular and functional characterisation of genes encoding Tre metabolising enzymes during pea seed development. In detail, we want to study tissue and cell-specific gene expression of Tre metabolism genes in seeds. Regulation of gene expression will be monitored in a set of unique transgenic pea seed models with defined changes of their metabolic state. Regulation of promoter activities from these genes will be analysed in response to nutrient/hormone treatments. In addition, we want to unravel the regulation of class II Tre-P synthases by searching for protein interacting partners. Finally, we are planning a functional analysis of Tre metabolism during pea seed development by transgenic inhibition of gene expression. Levels of Tre-6-P will be modified by ectopic expression of E. coli Tre-P synthase and Tre-P phosphatase. Transgenic seeds will be subjected to phenotypic analysis by monitoring growth parameters and seed composition. From this project, we expect a more profound insight into the regulation of sugar metabolism and storage product synthesis of pea seeds in particular and of crop seeds in general.

种子代谢必须与内源发育和外源环境因素协调。这被认为是通过 SnRK1、脱落酸和海藻糖 (Tre) 途径介导的糖信号传导来实现的。 Tre 和 Tre-6-P 是植物中的次要糖，但代表着整合和协调代谢与发育的重要信号。在这个项目中，我们计划对豌豆种子发育过程中编码 Tre 代谢酶的基因进行分子和功能表征。详细来说，我们想要研究种子中 Tre 代谢基因的组织和细胞特异性基因表达。将在一组独特的转基因豌豆种子模型中监测基因表达的调节，并确定其代谢状态的变化。将分析这些基因启动子活性对营养/激素治疗的反应的调节。此外，我们希望通过寻找蛋白质相互作用伙伴来解开 II 类 Tre-P 合酶的调控。最后，我们计划通过基因表达的转基因抑制对豌豆种子发育过程中的 Tre 代谢进行功能分析。 Tre-6-P 的水平将因大肠杆菌 Tre-P 合酶和 Tre-P 磷酸酶的异位表达而改变。将通过监测生长参数和种子组成对转基因种子进行表型分析。通过这个项目，我们期望对豌豆种子和一般作物种子的糖代谢和储存产物合成的调节有更深入的了解。