Time/space Patterns of Microsatellite DNA Variation in Relation to Patch Structure of Southern Ocean Krill, Euphausia Superba

微卫星DNA变异的时空模式与南大洋磷虾斑块结构的关系

基本信息

批准号：
0610789
负责人：
Ann Bucklin
金额：
$ 14.54万
依托单位：
University of Connecticut
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2005
资助国家：
美国
起止时间：
2005-12-15 至 2007-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0610789&HistoricalAwards=false
关键词：
Time space Patterns Microsatellite DNA

项目摘要

The proposed study will use genetic markers to examine population genetic diversity and structure of the Southern Ocean krill, Euphausia superba (Crustacea, Euphausiacea). This effort builds upon previous work by one of the project participants to develop these genetic markers and the appropriate protocols for E. superba. Molecular genetic analysis is planned for archived samples collected during Southern Ocean cruises to the Western Antarctic Peninsula (WAP) during 2001 and 2002. Temporal and spatial patterns of genetic diversity and structure will be described in the context of krill swarms, bathymetry of the collection region, and mesoscale ocean circulation. Specimens of E. superba will be identified from alcohol-preserved zooplankton samples. Krill from different cruises and diverse regions of the sampled domain will be used to screen 20 to 40 genetic markers to determine levels of genetic differences between krill collected in different regions and at different times of the year in the Southern Ocean. The long-term goal of this effort is to contribute to our understanding of the population dynamic processes determining recruitment and maintenance of Southern Ocean krill. Broader impacts for this effort include training of an undergraduate student; international collaboration in research and education with researchers and students in Europe; and new understanding of the genetic architecture of a pivotal species in a polar ecosystem that is likely to be impacted by global change.

拟议的研究将使用遗传标记来检查南大洋奎莉，Euphausia Superba（Crustacea，euphausiacea）的种群遗传多样性和结构。这项工作是基于项目参与者以前的工作，以开发这些遗传标记和E. Superba的适当协议。计划在2001年和2002年在南方海洋巡航（WAP）中收集到的归档样品的分子遗传分析。遗传多样性和结构的时间和空间模式将在磷虾群，收集区域的沐浴仪以及中镜海洋回收的背景下进行描述。 E. Superba的标本将从饮酒保存的浮游动物样品中鉴定出来。采样结构域的不同巡航和不同区域的磷虾将用于筛选20至40个遗传标记，以确定在不同地区和南大洋一年中不同地区收集的磷虾之间的遗传差异水平。这项努力的长期目标是为我们理解决定招募和维持南方海洋磷虾的人口动态过程。这项工作的更广泛影响包括对本科生的培训；与欧洲研究人员和学生在研究和教育方面的国际合作；以及对极性生态系统中关键物种的遗传结构的新理解可能会受到全球变化影响。