Dissertation Research: Phylogeny and Functional Evolution of High Affinity Nitrate Transporters in Fungi

论文研究：真菌中高亲和力硝酸盐转运蛋白的系统发育和功能进化

基本信息

批准号：
0608017
负责人：
David Hibbett
金额：
$ 1.2万
依托单位：
Clark University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2006
资助国家：
美国
起止时间：
2006-06-15 至 2008-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0608017&HistoricalAwards=false
关键词：
Dissertation Research Phylogeny Functional Evolution

项目摘要

Nitrate transporters deliver nutritional nitrogen to (among other organisms) mushroom forming fungi living in symbiosis with tree roots, yet how nitrate transporter genes evolve and their relative significance to forest ecology is not well understood. This project investigates how nitrate transporters have evolved and diversified in function during a mushroom's adaptation to its ecological niche. Two specific goals of the project are (1) to use DNA and amino acid sequence analysis to develop an hypothesis of the evolution of nitrate transporter genes in the fungi and improve our understanding of fungal taxonomy in some groups and (2) to analyze the patterns of nitrate transporter gene utilization by mushrooms from divergent nitrogen niches under an array of environmental conditions. This project addresses the importance of environmental nitrogen in the biodiversity and evolution of mushroom forming fungi with a specific focus on a group that forms symbiotic associations with trees. Nitrogen pollution is a broad ecological concern specifically implicated in the recent loss of diversity of these forest fungi. Such a loss threatens the resiliency and viability of the forest ecosystems that depend upon them directly. This project will provide a molecular framework for developing strategies of bioremediation using mushroom forming fungi to counter the impacts of nitrogen pollution.

硝酸盐转运蛋白将营养氮传递给（以及其他生物）与树根共生的蘑菇形成真菌，但硝酸盐转运蛋白基因如何进化及其对森林生态的相对意义尚不清楚。该项目研究了硝酸盐转运蛋白在蘑菇适应其生态位的过程中如何进化和功能多样化。该项目的两个具体目标是（1）利用 DNA 和氨基酸序列分析来提出真菌中硝酸盐转运蛋白基因进化的假设，并提高我们对某些类群真菌分类学的理解；（2）分析模式在一系列环境条件下，蘑菇对来自不同氮生态位的硝酸盐转运蛋白基因的利用。该项目探讨了环境氮在蘑菇形成真菌的生物多样性和进化中的重要性，特别关注与树木形成共生关系的群体。氮污染是一个广泛的生态问题，特别与最近这些森林真菌多样性的丧失有关。这种损失威胁到直接依赖于它们的森林生态系统的弹性和生存能力。该项目将为开发利用蘑菇形成真菌来对抗氮污染的影响的生物修复策略提供分子框架。