Orthogonal Modification of Biopolymers with Variable Peptide Sequences via Ultra-Rapid Covalent Modification

通过超快速共价修饰对具有可变肽序列的生物聚合物进行正交修饰

• 批准号: 175717199
• 负责人: Professor Dr. Christopher Barner-Kowollik
• 金额: --
• 依托单位: Institut für Chemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/175717199?language=en
• 关键词: Orthogonal; Modification; Biopolymers; Variable; Peptide

In the present proposal, ultra-rapid ambient temperature and catalyst-free coupling chemistry is investigated to be employed to bind monomer sequence-defined peptides onto biopolymer based surfaces. The aim will be to ultimately develop an access route to peptide modified biosubstrates, where the peptide segments constitute functions such as specific cell adhesion, biocidal activity or modulation of crystallization processes. The current proposal will demonstrate that extremely mild and catalyst-free conjugation strategies are ideally suited to ligate sensitive biomolecules to biological substrates.The overall aim of the project is subdivided into individual work packages, which have well-defined aims of their own. These are (i) the pre-functionalization and characterization of variable poly(saccharide) surfaces, e.g. cellulose, chitin, chitosan and hyaluronic acid systems, with highly electron deficient dithioesters, (ii) the establishment of a synthetic methodology to introduce dithioester or cyclopentadienyl moieties to supported peptides including model studies of the coupling of these in homogenous solution to synthetic polymers or soluble poly(saccharides), (iii) the functionalization of the electron deficient dithioester decorated poly(saccharide) surfaces with peptide sequences as well as the in-depth characterization of the generated surfaces, and (iv) the application of the developed ultra-rapid ambient temperature grafting procedure for the preparation of functional substrates.

在本提案中，研究了超优化的环境温度和无催化剂偶联化学的化学作用，以结合单体序列定义的肽与基于生物聚合物的表面结合。目的是最终开发通向肽修饰的生物基质的访问途径，在该肽段构成诸如特定细胞粘附，杀菌活性或结晶过程的调节之类的功能。当前的建议将表明，极度温和和无催化剂的共轭策略非常适合将敏感的生物分子与生物底物结合。这些是（i）可变聚（糖）表面的官能化和表征，例如纤维素，几丁质，壳聚糖和透明质酸系统，具有高度电子不足的依二硫代，（ii）建立一种合成方法，用于引入二硫代或环戊二烯基部分，以支持这些肽，包括这些肽，包括这些模型研究这些在合成Polumer或coluble或coluble或coluble或coluble或coluble或coluble con的研究中的模型研究聚（糖），（iii）具有肽序列的电子缺乏二硫酯装饰的聚（糖）表面以及生成的表面的深入表征，以及（iv）已发达的超增强环境的应用用于制备功能底物的温度移植程序。