High-Resolution CCD Camera and Luminometer for High-Throughput Luciferase-Based Analyses

用于基于荧光素酶的高通量分析的高分辨率 CCD 相机和发光计

• 批准号: 0500601
• 负责人: David Somers
• 金额: --
• 依托单位: Ohio State University Research Foundation -DO NOT USE
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0500601&HistoricalAwards=false
• 关键词: Resolution; CCD; Camera; Luminometer; Throughput

This award supports purchase of two systems to be used to localize and measure luciferase activity resulting from expression of chimeric reporter genes that consist of the luciferase gene fused to a promoter region of the genome. Such fusions are now routinely used as a convenient, though indirect, measure of gene regulation, and of the timing and localization of the expression of specific genes within an organism. One of the systems can acquire, process and analyze photons emitted by active luciferase. This system will be used primarily to follow time-course changes in gene expression in vivo, through the use of transgenic plants expressing specific promoter-luciferase reporter genes. The second system is a high throughput 96-well plate reader designed to allow rapid, automated measurements of luminescence in cell extracts. This instrument will be used for transient gene expression assays employing various cells lines. The two will be used by five investigators drawn from four academic departments. Their research includes studies of the molecular genetics of the circadian clock in Arabidopsis, the genetic regulation of sugar sensing in plants, the regulation of flavonoid biosynthesis and small molecular trafficking in maize, the molecular machinery of cell plate formation in plants, and the regulation of circadian clock gene transcription in mammalian cell culture. In addition, the equipment will be used in the research training of graduate postdoctoral students and incorporated into an existing undergraduate laboratory techniques course. The equipment will thus enhance student training at all levels in modern in vivo imaging and luminescence-based techniques.

该奖项支持购买两个系统，用于定位和测量由融合到基因组启动子区域的荧光素酶基因组成的嵌合报告基因表达产生的荧光素酶活性。 这种融合现在通常用作基因调控以及生物体内特定基因表达的时间和定位的一种方便但间接的测量方法。 其中一个系统可以采集、处理和分析活性荧光素酶发射的光子。 该系统将主要用于通过使用表达特定启动子-荧光素酶报告基因的转基因植物来跟踪体内基因表达的时间过程变化。第二个系统是高通量 96 孔板读数器，旨在快速、自动测量细胞提取物中的发光。该仪器将用于采用各种细胞系的瞬时基因表达测定。这两者将由来自四个学术部门的五名研究人员使用。他们的研究包括拟南芥生物钟的分子遗传学、植物糖传感的遗传调控、玉米类黄酮生物合成和小分子运输的调控、植物细胞板形成的分子机制以及哺乳动物细胞培养中的生物钟基因转录。 此外，该设备将用于博士后研究生的研究培训，并纳入现有的本科实验室技术课程。 因此，该设备将加强现代体内成像和发光技术各个级别的学生培训。