Interactions of the TCR co-receptors and p56LCK in an Ectothermic Model

变温模型中 TCR 共受体和 p56LCK 的相互作用

• 批准号: 0453924
• 负责人: John Hansen
• 金额: $ 3.4万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0453924&HistoricalAwards=false
• 关键词: Interactions; TCR; co; receptors; p56LCK

The vertebrate immune system serves to protect individuals from disease and neoplasm. It is composed of innate and adaptive characteristics that have been well-studied in mammals but very little is known about the processes governing the development of the immune repertoire in lower vertebrates, such as fish. Thus a fish model can provide an evolutionary perspective into the processes that have shaped the vertebrate immune system. Cytotoxic and helper T-cells are derived from a common T-cell precursor that commits to either of these two lineages after receiving specific signals in the thymus through a process known as T-cell development. Two different models have been put forth to explain T-cell development in mammals. It is the goal of this study to examine this process in rainbow trout to provide a comparative approach for examining the two models. This study will assess the pathways and signals that lead to development of the two T-cell subsets in fish. Specifically, this proposal will allow for the development of tools such as monoclonal antibodies that can be used to follow the developmental program that trout T-cells utilize during their development.This project will also serve to train and educate high school and undergraduate students in critical thought and current techniques in molecular biology and immunology. It will also show students the value of using comparative studies to solve fundamental paradigms in biology. Overall, the major objective of this project is to provide a basic understanding of the processes governing T-cell development in fish with a long-term goal of ascertaining the extent of conservation for this process in all vertebrates. These studies will have ramifications for research dealing the development of T-cells in mammals while also providing basic research and tools that can be applied to the preservation and maintenance of salmonid fish.

脊椎动物免疫系统可保护个体免受疾病和肿瘤的侵害。 它由哺乳动物中经过充分研究的先天和适应性特征组成，但对管理下脊椎动物（例如鱼）的免疫曲目发展的过程知之甚少。 因此，鱼类模型可以为塑造脊椎动物免疫系统的过程提供进化观点。细胞毒性和辅助T细胞源自常见的T细胞前体，该前体通过称为T细胞发育的过程在胸腺中接收特定信号后，在这两个谱系中都提交了这两个谱系中的任何一个。 已经提出了两个不同的模型来解释哺乳动物中的T细胞发育。 这项研究的目的是在彩虹鳟鱼中检查这一过程，以提供一种比较检查这两个模型的方法。 这项研究将评估导致鱼类中两个T细胞子集发展的途径和信号。 具体而言，该提案将允许开发工具，例如单克隆抗体，可用于遵循鳟鱼在开发过程中使用的开发计划。分子生物学和免疫学方面的思想和当前技术。它还将向学生展示使用比较研究解决生物学基本范式的价值。 总体而言，该项目的主要目的是对有关鱼类中T细胞开发的过程提供基本理解，其长期目标是确定所有脊椎动物的保护程度。 这些研究将对研究哺乳动物的T细胞开发的研究产生影响，同时还提供了可以应用于鲑鱼鱼的保存和维护的基础研究和工具。