SBIR Phase II: Membrane Protein Microarrays

SBIR II 期：膜蛋白微阵列

• 批准号: 0450262
• 负责人: Athena Guo
• 金额: --
• 依托单位: MICROSURFACES INC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0450262&HistoricalAwards=false
• 关键词: SBIR; Phase; II; Membrane; Protein

This Small Business Innovation Research (SBIR) Phase II project proposes to develop a product platform based on polymer cushion coated glass slides with controlled surface charge density for membrane protein microarray fabrication. The key technical objectives for Phase II research are : (a) to complete quantitative studies on surface charge density in the formation of supported phospholipid bilayer (SPB) from charged lipids , (b) to develop the chemistry for the grafting / adsorption of polymer cushions, (c) to measure the activities of membrane proteins in SPBs, and (d) to fabricate membrane protein microarrays based on surface pre-patterning using soft lithography techniques.The commercial application of this project will be in the area of protein microarrays for use in disease diagnostics and for drug discovery research. The proposed technology will enable development of therapeutics aimed at membrane protein targets.

这项小型企业创新研究（SBIR）II期项目建议开发一个基于聚合物垫涂层玻璃载玻片，具有控制膜蛋白微阵列制造的表面电荷密度。第二阶段研究的关键技术目标是：（a）完成对表面电荷密度的定量研究，以形成来自带电脂质的磷脂双层（SPB），（b）开发化学性，以基于跨层蛋白的聚合物垫子的植入 /吸附，以衡量跨度蛋白的活性（c）使用软光刻技术的预制。该项目的商业应用将在蛋白质微阵列领域用于疾病诊断和药物发现研究。拟议的技术将使针对膜蛋白靶标的治疗剂开发。