Collaborative Research: Metabolic Engineering of Saccharomyces cerevisiae for Enhanced Accumulation of Arsenic

合作研究：酿酒酵母代谢工程增强砷积累

• 批准号: 0422684
• 负责人: Nancy Da Silva
• 金额: --
• 依托单位: University of California-Irvine
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0422684&HistoricalAwards=false
• 关键词: Collaborative; Research; Metabolic; Engineering; Saccharomyces

DaSilva0422684The goal of this research is to metabolically engineer yeast cells for the adsorption of heavy metal arsenic. The idea is to manipulate the transporter genes for both import and export of the ionic form of the heavy metal, as well as manipulating the reduction system inside the cell. The proposed work will also utilize an integration system that the co-principal investigator has developed in her laboratory. This integration method allows the sequential integration of multiple cloned genes. Advantages of this system include the ability to insert precise numbers of the same or different genes (important for metabolic engineering) and integration at dispersed chromosomal locations, improving the stability of the insertions. The initial cloning effort will allow for some characterization for subsequent development of optimal strains for hyper accumulation of arsenic.

DaSilva0422684这项研究的目标是对酵母细胞进行代谢改造，以吸附重金属砷。 这个想法是操纵转运蛋白基因以导入和导出重金属离子形式，以及操纵细胞内的还原系统。 拟议的工作还将利用联合首席研究员在其实验室开发的集成系统。 这种整合方法允许多个克隆基因的顺序整合。 该系统的优点包括能够插入精确数量的相同或不同基因（对于代谢工程很重要）并在分散的染色体位置进行整合，从而提高插入的稳定性。 最初的克隆工作将为随后开发砷超积累的最佳菌株提供一些表征。