Proteomic and epigenetic alterations associated with plant-based diets and CVD

与植物性饮食和心血管疾病相关的蛋白质组学和表观遗传改变

基本信息

批准号：
10643149
负责人：
Hyunju Kim
金额：
$ 5.59万
依托单位：
JOHNS HOPKINS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-04-01 至 2023-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10643149
关键词：
Adherence Affect African American American Animals Area Atherosclerosis Risk in Communities Biological Biological Process Biology Blood Pressure Body mass index Cardiovascular Diseases Cardiovascular system Communities DNA Methylation Data Data Discovery Data Set Diet Dietary Practices Dietary intake Dimensions Disease Outcome Doctor of Philosophy Early identification Eating Edible Plants Elasticity Ensure Epidemiology Epigenetic Process Etiology European Event Five-Year Plans Food Framingham Heart Study Future Gene Expression Genes Goals Homeostasis Inflammation Inflammatory Knowledge Link Machine Learning Mentored Research Scientist Development Award Mentors Mentorship Metabolic Metabolism Methylation Methyltransferase Multi-Ethnic Study of Atherosclerosis Multiomic Data Nutritional Nutritional Study Pathogenesis Pathway interactions Pattern Phenotype Plants Prevention strategy Production Productivity Prospective Studies Prospective cohort Proteins Proteome Proteomics Public Health Schools Publishing Recommendation Research Research Personnel Research Proposals Research Training Risk Factors Risk Reduction Sample Size Statistical Models Techniques Technology Training Validation Vascular Diseases Vegan Diet adjudication aptamer cardiometabolic risk cardioprotection cardiovascular disorder prevention cardiovascular disorder risk career cohort collaborative environment dietary guidelines early detection biomarkers epigenetic marker experience follow-up good diet hands on research indexing metabolome metabolomics methylation pattern methylome middle age multidisciplinary multiple omics novel nutrition nutritional epidemiology prevent programs prospective protein biomarkers skills therapeutic target treatment strategy

项目摘要

Project Summary / Abstract Healthy diets reduce the risk of cardiovascular disease (CVD). Plant-based diets, which are comprised predominantly of plant foods and are low in animal products, are healthy dietary patterns that are associated with a lower risk of CVD. However, the precise mechanisms underlying plant-based diet-CVD associations are poorly understood. The present proposal aims to elucidate the mechanisms through which plant-based diets are associated with CVD using proteomics and epigenetics. Specific aims of the present proposal are to: 1) identify protein signatures of 4 plant-based diet indices [overall plant-based diet index (PDI), provegetarian diet, healthy plant-based diet index (hPDI), and unhealthy plant-based diet index (uPDI)], 2) evaluate the prospective associations between plant-based diet-related proteins and incident CVD, and 3) explore DNA methylation signatures of 4 plant-based diet indices. The proposed study will use the Atherosclerosis Risk in Communities (ARIC) study, a richly phenotyped community-based prospective study of middle-aged African American and European American with usual dietary intake, proteomics and DNA methylation data, and adjudicated CVD outcomes as the discovery data set. Framingham Heart Study and Multi-Ethnic Study of Atherosclerosis, well-characterized prospective cohorts with similar data, will be used for external validation. Completion of the aims will identify novel proteins, epigenetic markers, and pathways that are modifiable by plant-based diets. Such novel evidence will be the first step for developing biomarkers for early detection and identifying therapeutic targets of CVD. Hyunju Kim, PhD seeks a K01 Mentored Research Scientist Development Award to acquire essential skills, knowledge, and mentored research experience to prepare for a future career as an independent investigator with expertise in nutrition, multi-omics, and CVD. Dr. Kim has a track record of research productivity in the areas of nutritional epidemiology and nutritional metabolomics. However, Dr. Kim needs additional training in proteomics, epigenetics, and cardiovascular biology to achieve research independence. This research proposal details a five-year plan consisting of didactic coursework, hands-on research training, career enrichment programs under the primary mentorship of Dr. Casey Rebholz, PhD (expert in nutritional proteomics), and co-mentorship by Dr. Josef Coresh, MD, PhD (expert in proteomics and vascular disease), and Dr. Dan Arking, PhD (expert in epigenetics). The exceptional mentoring team in addition to external collaborators and advisors with multi-disciplinary expertise, and a highly collaborative environment at Johns Hopkins Bloomberg School of Public Health will ensure the successful transition of Dr. Kim into an independent investigator.

项目概要/摘要健康饮食可降低心血管疾病 (CVD) 的风险。以植物为基础的饮食，包括以植物性食品为主，动物性食品含量较低，是与健康饮食模式相关的 CVD 风险较低。然而，植物性饮食与 CVD 关联的确切机制是不太了解。本提案旨在阐明植物性饮食的机制使用蛋白质组学和表观遗传学将其与 CVD 相关联。本提案的具体目标是：1）确定 4 种植物性饮食指数的蛋白质特征 [总体植物性饮食指数 (PDI)、素食饮食、健康植物性饮食指数 (hPDI) 和不健康饮食植物性饮食指数（uPDI）]，2）评估植物性饮食相关因素之间的前瞻性关联蛋白质和 CVD 事件，以及 3) 探索 4 种植物性饮食指数的 DNA 甲基化特征。这拟议的研究将使用社区动脉粥样硬化风险 (ARIC) 研究，这是一项具有丰富表型的研究对中年非洲裔美国人和欧洲裔美国人进行的基于社区的前瞻性研究饮食摄入量、蛋白质组学和 DNA 甲基化数据以及判定的 CVD 结果作为发现数据放。弗雷明汉心脏研究和动脉粥样硬化的多种族研究，特征明确的前瞻性研究具有相似数据的队列将用于外部验证。完成目标将识别新的蛋白质，表观遗传标记以及可通过植物性饮食改变的途径。这些新颖的证据将是开发用于早期检测和识别 CVD 治疗靶点的生物标志物的第一步。 Hyunju Kim 博士寻求 K01 指导研究科学家发展奖以获得必要的知识技能、知识和指导研究经验，为未来的独立职业生涯做好准备具有营养、多组学和 CVD 专业知识的研究员。 Kim 博士拥有丰富的研究记录营养流行病学和营养代谢组学领域的生产力。然而，金博士需要蛋白质组学、表观遗传学和心血管生物学方面的额外培训，以实现研究独立性。该研究计划详细介绍了一个五年计划，包括教学课程、实践研究培训、在 Casey Rebholz 博士（营养学专家）的主要指导下开展职业充实计划蛋白质组学），并由 Josef Coresh 博士（蛋白质组学和血管疾病专家）共同指导，和 Dan Arking 博士（表观遗传学专家）。除了外部的优秀指导团队约翰斯拥有多学科专业知识的合作者和顾问以及高度协作的环境霍普金斯大学彭博公共卫生学院将确保金博士成功过渡为独立的研究者。